yam8+, a Schizosaccharomyces pombe gene, is a potential homologue of the Saccharomyces cerevisiae MID1 gene encoding a stretch-activated Ca2+-permeable channel

Yasushi Tasaka, Yuko Nakagawa, Chikara Sato, Masanobu Mino, Nobuyuki Uozumi, Norio Murata, Shoshi Muto, Hidetoshi Iida

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The Saccharomyces cerevisiae MID1 gene encodes a stretch-activated Ca2+-permeable channel. In a protein database, we found a Schizosaccharomyces pombe gene whose predicted protein shows 26% identical and 62% similar to the Mid1 channel in amino acid sequence. cDNA derived from this gene, designated yam8+, was isolated by reverse transcription-polymerase chain reaction (RT-PCR). Further analysis showed that the Yam8 protein consists of 486 amino acids and has 6 hydrophobic segments. The yam8+ cDNA, placed under the S. cerevisiae TDH3 promoter, partially complemented the mating pheromone-induced death (mid) phenotype of the S. cerevisiae mid1 mutant. The expression of the yam8+ cDNA in the midi mutant cells partially remediated the mid phenotype and resulted in a slight increase in Ca2+ uptake activity. These findings suggest that Yam8 is a potential homologue of Mid1. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)265-269
Number of pages5
JournalBiochemical and biophysical research communications
Volume269
Issue number1
DOIs
Publication statusPublished - 2000 Mar 5
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'yam8<sup>+</sup>, a Schizosaccharomyces pombe gene, is a potential homologue of the Saccharomyces cerevisiae MID1 gene encoding a stretch-activated Ca<sup>2+</sup>-permeable channel'. Together they form a unique fingerprint.

Cite this