Xyloglucan is not essential for the formation and integrity of the cellulose network in the primary cell wall regenerated from arabidopsis protoplasts

Hiroaki Kuki, Ryusuke Yokoyama, Takeshi Kuroha, Kazuhiko Nishitani

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The notion that xyloglucans (XG) play a pivotal role in tethering cellulose microfibrils in the primary cell wall of plants can be traced back to the first molecular model of the cell wall proposed in 1973, which was reinforced in the 1990s by the identification of Xyloglucan Endotransglucosylase/Hydrolase (XTH) enzymes that cleave and reconnect xyloglucan crosslinks in the cell wall. However, this tethered network model has been seriously challenged since 2008 by the identification of the Arabidopsis thaliana xyloglucan-deficient mutant (xxt1 xxt2), which exhibits functional cell walls. Thus, the molecular mechanism underlying the physical integration of cellulose microfibrils into the cell wall remains controversial. To resolve this dilemma, we investigated the cell wall regeneration process using mesophyll protoplasts derived from xxt1 xxt2 mutant leaves. Imaging analysis revealed only a slight difference in the structure of cellulose microfibril network between xxt1 xxt2 and wild-type (WT) protoplasts. Additionally, exogenous xyloglucan application did not alter the cellulose deposition patterns or mechanical stability of xxt1 xxt2 mutant protoplasts. These results indicate that xyloglucan is not essential for the initial assembly of the cellulose network, and the cellulose network formed in the absence of xyloglucan provides sufficient tensile strength to the primary cell wall regenerated from protoplasts.

Original languageEnglish
Article number629
JournalPlants
Volume9
Issue number5
DOIs
Publication statusPublished - 2020

Keywords

  • Arabidopsis thaliana
  • Cellulose microfibril
  • Image analysis
  • Primary cell wall
  • Protoplast
  • Xxt1 xxt2
  • Xyloglucan

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Plant Science

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