X-ray-induced mutations in Escherichia coli K-12 strains with altered DNA polymerase I activities

Yuki Nagata, Masakado Kawata, Jun Ichiro Komura, Tetsuya Ono, Kazuo Yamamoto

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Spectra of ionizing radiation mutagenesis were determined by sequencing X-ray-induced endogenous tonB gene mutations in Escherichia coli polA strains. We used two polA alleles, the polA1 mutation, defective for Klenow domain, and the polA107 mutation, defective for flap domain. We demonstrated that irradiation of 75 and 50 Gy X-rays could induce 3.8- and 2.6-fold more of tonB mutation in polA1 and polA107 strains, respectively, than spontaneous level. The radiation induced spectrum of 51 tonB mutations in polA1 and 51 in polA107 indicated that minus frameshift, A:T → T:A transversion and G:C → T:A transversion were the types of mutations increased. Previously, we have reported essentially the same X-ray-induced tonB mutation spectra in the wild-type strain. These results indicate that (1) X-rays can induce minus frameshift, A:T → T:A transversion and G:C → T:A transversion in E. coli and (2) presence or absence of polymerase I (PolI) of E. coli does not have any effects on the process of X-ray mutagenesis.

Original languageEnglish
Pages (from-to)93-103
Number of pages11
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume528
Issue number1-2
DOIs
Publication statusPublished - 2003 Jul 25

Keywords

  • Minus frameshift
  • PolI Klenow domain
  • PolI flap domain
  • Transversions
  • X-ray mutagenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

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