X-ray computed tomography imaging of a tumor with high sensitivity using gold nanoparticles conjugated to a cancer-specific antibody via polyethylene glycol chains on their surface

Tomohiko Nakagawa, Kohsuke Gonda, Takashi Kamei, Liman Cong, Yoh Hamada, Narufumi Kitamura, Hiroshi Tada, Takanori Ishida, Takuji Aimiya, Naoko Furusawa, Yasushi Nakano, Noriaki Ohuchi

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Contrast agents are often used to enhance the contrast of X-ray computed tomography (CT) imaging of tumors to improve diagnostic accuracy. However, because the iodine-based contrast agents currently used in hospitals are of low molecular weight, the agent is rapidly excreted from the kidney or moves to extravascular tissues through the capillary vessels, depending on its concentration gradient. This leads to nonspecific enhancement of contrast images for tissues. Here, we created gold (Au) nanoparticles as a new contrast agent to specifically image tumors with CT using an enhanced permeability and retention (EPR) effect. Au has a higher X-ray absorption coefficient than does iodine. Au nanoparticles were supported with polyethylene glycol (PEG) chains on their surface to increase the blood retention and were conjugated with a cancer-specific antibody via terminal PEG chains. The developed Au nanoparticles were injected into tumor-bearing mice, and the distribution of Au was examined with CT imaging, transmission electron microscopy, and elemental analysis using inductively coupled plasma optical emission spectrometry. The results show that specific localization of the developed Au nanoparticles in the tumor is affected by a slight difference in particle size and enhanced by the conjugation of a specific antibody against the tumor.

Original languageEnglish
Pages (from-to)387-397
Number of pages11
JournalScience and Technology of Advanced Materials
Volume17
Issue number1
DOIs
Publication statusPublished - 2016 Jan 1

Keywords

  • CT
  • contrast agent
  • gold
  • nanoparticle
  • tumor

ASJC Scopus subject areas

  • Materials Science(all)

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