WOX13-like genes are required for reprogramming of leaf and protoplast cells into stem cells in the moss physcomitrella patens

Keiko Sakakibara, Pascal Reisewitz, Tsuyoshi Aoyama, Thomas Friedrich, Sayuri Ando, Yoshikatsu Sato, Yosuke Tamada, Tomoaki Nishiyama, Yuji Hiwatashi, Tetsuya Kurata, Masaki Ishikawa, Hironori Deguchi, Stefan A. Rensing, Wolfgang Werr, Takashi Murata, Mitsuyasu Hasebe, Thomas Laux

    Research output: Contribution to journalArticle

    67 Citations (Scopus)

    Abstract

    Many differentiated plant cells can dedifferentiate into stem cells, reflecting the remarkable developmental plasticity of plants. In the moss Physcomitrella patens, cells at the wound margin of detached leaves become reprogrammed into stem cells. Here, we report that two paralogous P. patens WUSCHEL-related homeobox 13-like (PpWOX13L) genes, homologs of stem cell regulators in flowering plants, are transiently upregulated and required for the initiation of cell growth during stem cell formation. Concordantly, Δppwox13l deletion mutants fail to upregulate genes encoding homologs of cell wall loosening factors during this process. During the moss life cycle, most of the Δppwox13l mutant zygotes fail to expand and initiate an apical stem cell to form the embryo. Our data show that PpWOX13L genes are required for the initiation of cell growth specifically during stem cell formation, in analogy to WOX stem cell functions in seed plants, but using a different cellular mechanism.

    Original languageEnglish
    Pages (from-to)1660-1670
    Number of pages11
    JournalDevelopment (Cambridge)
    Volume141
    Issue number8
    DOIs
    Publication statusPublished - 2014 Apr

    Keywords

    • Physcomitrella
    • Stem cell
    • WOX

    ASJC Scopus subject areas

    • Molecular Biology
    • Developmental Biology

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  • Cite this

    Sakakibara, K., Reisewitz, P., Aoyama, T., Friedrich, T., Ando, S., Sato, Y., Tamada, Y., Nishiyama, T., Hiwatashi, Y., Kurata, T., Ishikawa, M., Deguchi, H., Rensing, S. A., Werr, W., Murata, T., Hasebe, M., & Laux, T. (2014). WOX13-like genes are required for reprogramming of leaf and protoplast cells into stem cells in the moss physcomitrella patens. Development (Cambridge), 141(8), 1660-1670. https://doi.org/10.1242/dev.097444