Wnt5a attenuates Wnt3a-induced alkaline phosphatase expression in dental follicle cells

Yukihiko Sakisaka, Masahiro Tsuchiya, Takashi Nakamura, Masato Tamura, Hidetoshi Shimauchi, Eiji Nemoto

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Wnt signaling regulates multiple cellular events such as cell proliferation, differentiation, and apoptosis through β-catenin-dependent canonical and β-catenin-independent noncanonical pathways. Canonical Wnt/β-catenin signaling can promote the differentiation of dental follicle cells, putative progenitor cells for cementoblasts, osteoblasts, and periodontal ligament cells, toward a cementoblast/osteoblast phenotype during root formation, but little is known about the biological significance of noncanonical Wnt signaling in this process. We identified the expression of Wnt5a, a representative noncanonical Wnt ligand, in tooth root lining cells (i.e. precementoblasts/cementoblasts) and dental follicle cells during mouse tooth root development, as assessed by immunohistochemistry. Silencing expression of the Wnt5a gene in a dental follicle cell line resulted in enhancement of the Wnt3a (a representative canonical Wnt ligand)-mediated increase in alkaline phosphatase (ALP) expression. Conversely, treatment with recombinant Wnt5a inhibited the increase in ALP expression, suggesting that Wnt5a signaling functions as a negative regulator of canonical Wnt-mediated ALP expression of dental follicle cells. Wnt5a did not affect the nuclear translocation of β-catenin as well as β-catenin-mediated transcriptional activation of T-cell factor (Tcf) triggered by Wnt3a, suggesting that Wnt5a inhibits the downstream part of the β-catenin-Tcf pathway. These findings suggest the existence of a feedback mechanism between canonical and noncanonical Wnt signaling during the differentiation of dental follicle cells.

Original languageEnglish
Pages (from-to)85-93
Number of pages9
JournalExperimental Cell Research
Volume336
Issue number1
DOIs
Publication statusPublished - 2015 Jan 1

Keywords

  • Alkaline phosphatase
  • Cell differentiation
  • Dental follicle cells
  • Wnt3a
  • Wnt5a

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Wnt5a attenuates Wnt3a-induced alkaline phosphatase expression in dental follicle cells'. Together they form a unique fingerprint.

  • Cite this