WIP is a chaperone for Wiskott-Aldrich syndrome protein (WASP)

Miguel A. De La Fuente, Yoji Sasahara, Marco Calamito, Inés M. Antón, Abdallah Elkhal, Maria D. Gallego, Koduru Suresh, Katherine Siminovitch, Hans D. Ochs, Kenneth C. Anderson, Fred S. Rosen, Raif S. Geha, Narayanaswamy Ramesh

Research output: Contribution to journalArticlepeer-review

115 Citations (Scopus)

Abstract

Wiskott-Aldrich syndrome protein (WASP) is in a complex with WASP-interacting protein (WIP). WASP levels, but not mRNA levels, were severely diminished in T cells from WIP-/- mice and were increased by introduction of WIP in these cells. The WASP binding domain of WIP was shown to protect WASP from degradation by calpain in vitro. Treatment with the proteasome inhibitors MG132 and bortezomib increased WASP levels in T cells from WIP -/- mice and in T and B lymphocytes from two WAS patients with missense mutations (R86H and T45M) that disrupt WIP binding. The calpain inhibitor calpeptin increased WASP levels in activated T and B cells from the WASP patients, but not in primary T cells from the patients or from WIP -/- mice. Despite its ability to increase WASP levels proteasome inhibition did not correct the impaired IL-2 gene expression and low F-actin content in T cells from the R86H WAS patient. These results demonstrate that WIP stabilizes WASP and suggest that it may also be important for its function.

Original languageEnglish
Pages (from-to)926-931
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number3
DOIs
Publication statusPublished - 2007 Jan 16

Keywords

  • Proteasome
  • T cells

ASJC Scopus subject areas

  • General

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