TY - JOUR
T1 - WIP is a chaperone for Wiskott-Aldrich syndrome protein (WASP)
AU - De La Fuente, Miguel A.
AU - Sasahara, Yoji
AU - Calamito, Marco
AU - Antón, Inés M.
AU - Elkhal, Abdallah
AU - Gallego, Maria D.
AU - Suresh, Koduru
AU - Siminovitch, Katherine
AU - Ochs, Hans D.
AU - Anderson, Kenneth C.
AU - Rosen, Fred S.
AU - Geha, Raif S.
AU - Ramesh, Narayanaswamy
PY - 2007/1/16
Y1 - 2007/1/16
N2 - Wiskott-Aldrich syndrome protein (WASP) is in a complex with WASP-interacting protein (WIP). WASP levels, but not mRNA levels, were severely diminished in T cells from WIP-/- mice and were increased by introduction of WIP in these cells. The WASP binding domain of WIP was shown to protect WASP from degradation by calpain in vitro. Treatment with the proteasome inhibitors MG132 and bortezomib increased WASP levels in T cells from WIP -/- mice and in T and B lymphocytes from two WAS patients with missense mutations (R86H and T45M) that disrupt WIP binding. The calpain inhibitor calpeptin increased WASP levels in activated T and B cells from the WASP patients, but not in primary T cells from the patients or from WIP -/- mice. Despite its ability to increase WASP levels proteasome inhibition did not correct the impaired IL-2 gene expression and low F-actin content in T cells from the R86H WAS patient. These results demonstrate that WIP stabilizes WASP and suggest that it may also be important for its function.
AB - Wiskott-Aldrich syndrome protein (WASP) is in a complex with WASP-interacting protein (WIP). WASP levels, but not mRNA levels, were severely diminished in T cells from WIP-/- mice and were increased by introduction of WIP in these cells. The WASP binding domain of WIP was shown to protect WASP from degradation by calpain in vitro. Treatment with the proteasome inhibitors MG132 and bortezomib increased WASP levels in T cells from WIP -/- mice and in T and B lymphocytes from two WAS patients with missense mutations (R86H and T45M) that disrupt WIP binding. The calpain inhibitor calpeptin increased WASP levels in activated T and B cells from the WASP patients, but not in primary T cells from the patients or from WIP -/- mice. Despite its ability to increase WASP levels proteasome inhibition did not correct the impaired IL-2 gene expression and low F-actin content in T cells from the R86H WAS patient. These results demonstrate that WIP stabilizes WASP and suggest that it may also be important for its function.
KW - Proteasome
KW - T cells
UR - http://www.scopus.com/inward/record.url?scp=33846526209&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846526209&partnerID=8YFLogxK
U2 - 10.1073/pnas.0610275104
DO - 10.1073/pnas.0610275104
M3 - Article
C2 - 17213309
AN - SCOPUS:33846526209
VL - 104
SP - 926
EP - 931
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 3
ER -