Whole-plant growth and N utilization in transgenic rice plants with increased or decreased rubisco content under different CO 2 partial pressures

Emi Sudo, Yuji Suzuki, Amane Makino

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) strongly limits photosynthesis at lower CO2concentration [CO2] whereas 10 Rubisco limitation is cancelled by elevated [CO2]. Therefore, increase or reduction in Rubisco content by transformation with a sense or an antisense RBCS construct are expected to alter the biomass production under different CO2levels. RBCS-sense (125% Rubisco of wild-type) and -antisense (35% Rubisco of wild-type) rice (Oryza sativa L.) plants were grown for 63 days at three different CO2levels: low [CO2] (28 Pa), normal [CO2] (40 Pa) and elevated [CO2] (120 Pa). The biomass of RBCS-sense plants was 32% and 15% greater at low [CO2] and normal [CO2] than that of the wild-type plants, respectively, but did not differ at elevated [CO2]. Conversely, the biomass of RBCS-antisense plants was the smallest at low [CO2]. Thus, overproduction of Rubisco was effective for biomass production at low [CO2]. Greater biomass production at low [CO2] in RBCS-sense plants was caused by an increase in the net assimilation rate, and associated with an increase in the amount of N uptake. Furthermore, Rubisco overproduction in RBCS-sense plants was also promoted at low [CO2]. Although it seems that low [CO2]-growth additionally stimulates the effect of RBCS overexpression, such a phenomenon observed at low [CO2] was mediated through an increase in total leaf N content. Thus, the dependence of the growth improvement in RBCS-sense rice on growth [CO2] was closely related to the degree of Rubisco overproduction which was accompanied not only by leaf N content but also by whole plant N content.

Original languageEnglish
Pages (from-to)1905-1911
Number of pages7
JournalPlant and Cell Physiology
Volume55
Issue number11
DOIs
Publication statusPublished - 2014 Jun 27

Keywords

  • Biomass
  • Low and elevated [CO]
  • Nitrogen
  • Oryza sativa
  • RBCS
  • Rubisco overproduction

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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