TY - JOUR
T1 - Whole mitochondrial genome sequencing and re-examination of a cytoplasmic male sterility-associated gene in boro-taichung-type cytoplasmic male sterile rice
AU - Kazama, Tomohiko
AU - Toriyama, Kinya
N1 - Funding Information:
This study was partly supported by grants from the Ministry of Education, Culture, Sports, Science and Technology, Japan (http://www.mext.go. jp/english/, grant number 24117502 to K.T.), Japan Society for the Promotion of Science (http://www. jsps.go.jp/english/index.html, grant numbers 23380002, 26292002 to K.T. and 16H06182 to T. K.), the Ministry of Agriculture Forestry and Fishery, Japan (http://www.maff.go.jp/e/index.html, Technology Research Promotion Program for the Agriculture, Forestry, Fisheries and Food Industry, grant number 26010A to K.T.), and Council for Science, Technology and Innovation (http://www8. cao.go.jp/cstp/english/, Cross-ministerial Strategic Innovation Promotion Program (SIP), " Technologies for creating next-generation agriculture, forestry and fisheries" (funding agency: Bio-oriented Technology Research Advancement Institution, NARO; http:// www.naro.affrc.go.jp/english/brain/) to K.T.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2016 Kazama, Toriyama. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2016/7
Y1 - 2016/7
N2 - Nuclear genome substitutions between subspecies can lead to cytoplasmic male sterility (CMS) through incompatibility between nuclear and mitochondrial genomes. Boro-Taichung (BT)-type CMS rice was obtained by substituting the nuclear genome of Oryza sativa subsp. indica cultivar Chinsurah Boro II with that of Oryza sativa subsp. japonica cultivar Taichung 65. In BT-type CMS rice, the mitochondrial gene orf79 is associated with male sterility. A complete sequence of the Boro-type mitochondrial genome responsible for BT-type CMS has not been determined to date. Here, we used pyrosequencing to construct the Boro-type mitochondrial genome. The contiguous sequences were assembled into five circular DNA molecules, four of which could be connected into a single circle. The two resulting subgenomic circles were unable to form a reliable master circle, as recombination between them was scarcely detected. We also found an unequal abundance of DNA molecules for the two loci of atp6. These results indicate the presence of multi-partite DNA molecules in the Borotype mitochondrial genome. Expression patterns were investigated for Boro-type mitochondria-specific orfs, which were not found in the mitochondria from the standard japonica cultivar Nipponbare. Restorer of fertility 1 (RF1)-dependent RNA processing has been observed in orf79-containing RNA but was not detected in other Boro-type mitochondriaspecific orfs, supporting the conclusion that orf79 is a unique CMS-associated gene in Boro-type mitochondria.
AB - Nuclear genome substitutions between subspecies can lead to cytoplasmic male sterility (CMS) through incompatibility between nuclear and mitochondrial genomes. Boro-Taichung (BT)-type CMS rice was obtained by substituting the nuclear genome of Oryza sativa subsp. indica cultivar Chinsurah Boro II with that of Oryza sativa subsp. japonica cultivar Taichung 65. In BT-type CMS rice, the mitochondrial gene orf79 is associated with male sterility. A complete sequence of the Boro-type mitochondrial genome responsible for BT-type CMS has not been determined to date. Here, we used pyrosequencing to construct the Boro-type mitochondrial genome. The contiguous sequences were assembled into five circular DNA molecules, four of which could be connected into a single circle. The two resulting subgenomic circles were unable to form a reliable master circle, as recombination between them was scarcely detected. We also found an unequal abundance of DNA molecules for the two loci of atp6. These results indicate the presence of multi-partite DNA molecules in the Borotype mitochondrial genome. Expression patterns were investigated for Boro-type mitochondria-specific orfs, which were not found in the mitochondria from the standard japonica cultivar Nipponbare. Restorer of fertility 1 (RF1)-dependent RNA processing has been observed in orf79-containing RNA but was not detected in other Boro-type mitochondriaspecific orfs, supporting the conclusion that orf79 is a unique CMS-associated gene in Boro-type mitochondria.
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U2 - 10.1371/journal.pone.0159379
DO - 10.1371/journal.pone.0159379
M3 - Article
C2 - 27414645
AN - SCOPUS:84978437466
VL - 11
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 7
M1 - e0159379
ER -