What is critical for plant thermogenesis? Differences in mitochondrial activity and protein expression between thermogenic and non-thermogenic skunk cabbages

Yasuko Ito-Inaba, Yamato Hida, Takehito Inaba

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Thermogenesis during the blooming of inflorescence is found in several but not all aroids. To understand what is critical for thermogenesis, we investigated the difference between thermogenic and non-thermogenic skunk cabbages (Symplocarpus renifolius and Lysichiton camtschatcensis), which are closely related in morphology and phylogeny. Critical parameters of mitochondrial biogenesis, including density, respiratory activity, and protein expression were compared between these two species. Mitochondrial density, respiratory activity, and the amount of alternative oxidase (AOX) in L. camtschatcensis spadix mitochondria were lower than in S. renifolius spadix mitochondria, while the level of uncoupling protein (UCP) was higher. AOX and UCP mRNAs in L. camtschatcensis were constitutively expressed in various tissues, such as the spadix, the spathe, the stalk, and the leaves. cDNA encoding two putative thermogenic proteins, AOX and UCP were isolated from L. camtschatcensis, and their primary structure was analyzed by multiple alignment and phylogenetic tree reconstruction. AOX and UCP protein of two the skunk cabbage species are closely related in structure, compared with other isoforms in thermogenic plants. Our results suggest that mitochondrial density, respiratory activity, and protein expression, rather than the primary structure of AOX or UCP proteins, may play critical roles in thermogenesis in plants.

Original languageEnglish
Pages (from-to)121-130
Number of pages10
JournalPlanta
Volume231
Issue number1
DOIs
Publication statusPublished - 2009 Jan
Externally publishedYes

Keywords

  • Alternative oxidase
  • Lysichiton
  • Mitochondrial density
  • Respiration
  • Symplocarpus
  • Uncoupling protein

ASJC Scopus subject areas

  • Genetics
  • Plant Science

Fingerprint Dive into the research topics of 'What is critical for plant thermogenesis? Differences in mitochondrial activity and protein expression between thermogenic and non-thermogenic skunk cabbages'. Together they form a unique fingerprint.

Cite this