WGA lectin binding sites of the apical surface of corti epithelium: Enhancement by back-scattered electron imaging in guinea-pig inner ear

Yukio Katori, Akira Tonosaki, Tomonori Takasaka

Research output: Contribution to journalReview article

5 Citations (Scopus)

Abstract

The apical surface of the guinea-pig organ of Corti was examined with SEM after WGA-lectin histochemistry which has a binding specificity for sialic acid and N-acetyl-glucosamine. The contrast of colloidal gold markers were particularly enhanced by back-scattered electron imaging. WGA lectin showed remarkable preference for the microvilli as well as flat surface areas in the supporting cells. Stereociliary surfaces and intersteriociliary connections of the sensory hair cell were strongly labelled, whilst only rarely the markers were found in the flat areas of the apical surface of the hair cell. The connections, which are known to be involved in the initiation of the mechanoelectrical transduction, should consist of sialic acid and/or N-acetyl-glucosamine.

Original languageEnglish
Pages (from-to)207-212
Number of pages6
JournalJournal of Electron Microscopy
Volume45
Issue number3
DOIs
Publication statusPublished - 1996

Keywords

  • Back-scattered electron imaging
  • Corti epithelium
  • Hair cell
  • SEM
  • WGA lectin

ASJC Scopus subject areas

  • Instrumentation

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