TY - JOUR
T1 - Wax D of Mycobacterium tuberculosis induced osteomyelitis accompanied by reactive bone formation in Buffalo rats
AU - Kawabata, Yoshihiro
AU - Semba, Ichiro
AU - Hirayama, Yoshikazu
AU - Koga, Toshitaka
AU - Nagao, Shigeki
AU - Takada, Haruhiko
N1 - Funding Information:
The authors are grateful to Professor M. Kitano (Kagoshima University Dental School) for his continuing interest and support during this work. We also thank Professors H. Nakashima and T. Noikura (Kagoshima University Dental School) for their stimulating suggestions and encouragement. We are indebted to H. Kataoka (Kagoshima University Dental School) for excellent technical advice and assistance, and D. Mrozek (Medical English Service, Kyoto, Japan) for reviewing this paper. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan (08771581).
PY - 1998/12
Y1 - 1998/12
N2 - A suspension of heat-killed Mycobacterium tuberculosis in liquid paraffin has been reported to induce foot swelling accompanied by new bone formation in Buffalo (BUF) rats, which are low responders to the induction of adjuvant arthritis. In the present study, we found that wax D, a mycobacterial cell wall peptidoglycan fragment-arabinogalactan-mycolic acid complex, was an effective component of this bacterium for the induction of osteomyelitis accompanied by reactive bone formation in BUF rats. Chronic inflammation was produced in BUF rats by a single subcutaneous injection of wax D suspended in liquid paraffin. Other Mycobacterium species and Gordona bronchialis were also capable of inducing this reaction. Other bacterial cells including the acid-fast bacteria Nocardia and Rhodococcus, purified cell walls and peptidoglycans from Lactobacillus plantarum, wax C, cord factor, arabinogalactan and mycolic acid prepared from M. tuberculosis were inactive in this respect. In addition, when wax D was administered as a water-in-oil emulsion (Freund's type adjuvant), bone formation scarcely occurred in BUF rats. In Fisher (F344) and Wistar rats, both of which are responder strains to adjuvant arthritis, wax D in liquid paraffin did not induce bone formation. Copyright (C) 1998 Federation of European Microbiological Societies.
AB - A suspension of heat-killed Mycobacterium tuberculosis in liquid paraffin has been reported to induce foot swelling accompanied by new bone formation in Buffalo (BUF) rats, which are low responders to the induction of adjuvant arthritis. In the present study, we found that wax D, a mycobacterial cell wall peptidoglycan fragment-arabinogalactan-mycolic acid complex, was an effective component of this bacterium for the induction of osteomyelitis accompanied by reactive bone formation in BUF rats. Chronic inflammation was produced in BUF rats by a single subcutaneous injection of wax D suspended in liquid paraffin. Other Mycobacterium species and Gordona bronchialis were also capable of inducing this reaction. Other bacterial cells including the acid-fast bacteria Nocardia and Rhodococcus, purified cell walls and peptidoglycans from Lactobacillus plantarum, wax C, cord factor, arabinogalactan and mycolic acid prepared from M. tuberculosis were inactive in this respect. In addition, when wax D was administered as a water-in-oil emulsion (Freund's type adjuvant), bone formation scarcely occurred in BUF rats. In Fisher (F344) and Wistar rats, both of which are responder strains to adjuvant arthritis, wax D in liquid paraffin did not induce bone formation. Copyright (C) 1998 Federation of European Microbiological Societies.
KW - Bone formation
KW - Buffalo rat
KW - Cell wall
KW - Mycobacterium tuberculosis
KW - Osteomyelitis
KW - Wax D
UR - http://www.scopus.com/inward/record.url?scp=0032435035&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032435035&partnerID=8YFLogxK
U2 - 10.1016/S0928-8244(98)00100-X
DO - 10.1016/S0928-8244(98)00100-X
M3 - Article
C2 - 9879920
AN - SCOPUS:0032435035
VL - 22
SP - 293
EP - 302
JO - Pathogens and Disease
JF - Pathogens and Disease
SN - 2049-632X
IS - 4
ER -