W-heterochromatin of chicken; its unusual DNA components, late replication, and chromatin structure

Noriyuki Suka, Yoshinobu Shinohara, Yasushi Saitoh, Hisato Saitoh, Kohei Ohtomo, Masahiko Harata, Edward Shpigelman, Shigeki Mizuno

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31 Citations (Scopus)

Abstract

About 65% of DNA in the chicken W chromosome has been shown to consist of XhoI and EcoRI family repetitive sequences. These sequences showed remarkable delay in the electrophoretic mobility at low temperature on a polyacrylamide gel. Three dimensional structures of the 0.7-kb XhoI and the 1.2-kb EcoRI family repeating units were estimated to be irregular solenoids using a computer program based on wedge angles of all the 16 dinucleotide steps. Fluorescence in situ hybridization demonstrated that these two family sequences were localized in a major heterochromatic body in an interphase nucleus. Incorporation of bromodeoxyuridine into the W chromosome in the synchronous culture of MSB-1 cells occurred about 1 h later than the peak of S phase. The chromatin structure formed along XhoI and EcoRI family sequences was suggested to be different from the total chromatin or chromatin containing the β-actin gene sequence in that the linker DNA lengths of the former were significantly longer. Fractionation of the HaeIII-digested MSB-1 nuclei yielded a chromatin fraction in which XhoI family sequences were partially enriched. Several DNA-binding proteins showing higher affinity for the XhoI family sequence were present in this fraction.

Original languageEnglish
Pages (from-to)93-105
Number of pages13
JournalGenetica
Volume88
Issue number2-3
DOIs
Publication statusPublished - 1993 Jun 1

Keywords

  • chicken W chromosome
  • curved repetitive DNA
  • heterochromatin
  • late replication
  • nucleosome

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics
  • Plant Science
  • Insect Science

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  • Cite this

    Suka, N., Shinohara, Y., Saitoh, Y., Saitoh, H., Ohtomo, K., Harata, M., Shpigelman, E., & Mizuno, S. (1993). W-heterochromatin of chicken; its unusual DNA components, late replication, and chromatin structure. Genetica, 88(2-3), 93-105. https://doi.org/10.1007/BF02424466