TY - JOUR
T1 - Voltage and Ca2+-activated K+ channel in baso-lateral acinar cell membranes of mammalian salivary glands
AU - Maruyama, Yoshio
AU - Gallacher, D. V.
AU - Petersen, O. H.
PY - 1983/12/1
Y1 - 1983/12/1
N2 - Nervous or hormonal stimulation of many exocrine glands evokes release of cellular K+ (ref. 1), as originally demonstrated in mammalian salivary glands2,3, and is associated with a marked increase in membrane conductance1,4,5. We now demonstrate directly, by using the patch-clamp technique6, the existence of a K+ channel with a large conductance localized in the basolateral plasma membranes of mouse and rat salivary gland acinar cells. The K+ channel has a conductance of ∼250 pS in the presence of high K+ solutions on both sides of the membrane. Although mammalian exocrine glands are believed not to possess voltage-activated channels1,7, the probability of opening the salivary gland K+ channel was increased by membrane depolarization. The frequency of channel opening, particularly at higher membrane potentials, was increased markedly by elevating the internal ionized Ca2+ concentration, as previously shown for high-conductance K+ channels from cells of neural origin8-10. The Ca2+ and voltage-activated K+ channel explains the marked cellular K + release that is characteristically observed when salivary glands are stimulated to secrete.
AB - Nervous or hormonal stimulation of many exocrine glands evokes release of cellular K+ (ref. 1), as originally demonstrated in mammalian salivary glands2,3, and is associated with a marked increase in membrane conductance1,4,5. We now demonstrate directly, by using the patch-clamp technique6, the existence of a K+ channel with a large conductance localized in the basolateral plasma membranes of mouse and rat salivary gland acinar cells. The K+ channel has a conductance of ∼250 pS in the presence of high K+ solutions on both sides of the membrane. Although mammalian exocrine glands are believed not to possess voltage-activated channels1,7, the probability of opening the salivary gland K+ channel was increased by membrane depolarization. The frequency of channel opening, particularly at higher membrane potentials, was increased markedly by elevating the internal ionized Ca2+ concentration, as previously shown for high-conductance K+ channels from cells of neural origin8-10. The Ca2+ and voltage-activated K+ channel explains the marked cellular K + release that is characteristically observed when salivary glands are stimulated to secrete.
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U2 - 10.1038/302827a0
DO - 10.1038/302827a0
M3 - Article
C2 - 6302513
AN - SCOPUS:0020646336
VL - 302
SP - 827
EP - 829
JO - Nature
JF - Nature
SN - 0028-0836
IS - 5911
ER -