Vitellogenin Synthesis in the Ovary of Scallop, Patinopecten yessoensis: Control by Estradiol-17β and the Central Nervous System

Makoto Osada, Teruki Takamura, Haruko Sato, Katsuyoshi Mori

Research output: Contribution to journalArticlepeer-review

103 Citations (Scopus)

Abstract

Elucidation of a profile of scallop vitellin formation associated with oogenesis and its endocrine control, and identification of a vitellogenin synthesizing site were immunologically undertaken by using anti-scallop Vn serum. Vn content increased during ovarian growth and accounted for more than 80% of the watersoluble protein of the ovary at the mature stage. In vivo injection of estradiol-17β (E2) resulted in an increase in Vn content in the ovary. In vitro accumulation of Vn in the ovarian tissue was promoted with E2 and a vitellogenesis promoting factor (VPF) from cerebral plus pedal ganglion which was heat stable, less than MW 10,000 and trypsin/ chymotrypsin resistant. Estrogen receptor (ER)-like immunoreactivity was found in the growing oocyte and the auxiliary cell in close contact with growing oocytes, in which Vn immunoreactivity was also found. It is suggested that the vitellogenin synthesis occurred inside the ovary, especially in the auxiliary cell, and is controled by E2 and VPF via ER.

Original languageEnglish
Pages (from-to)172-179
Number of pages8
JournalJournal of Experimental Zoology Part A: Comparative Experimental Biology
Volume299
Issue number2
DOIs
Publication statusPublished - 2003 Oct 1

ASJC Scopus subject areas

  • Animal Science and Zoology

Fingerprint

Dive into the research topics of 'Vitellogenin Synthesis in the Ovary of Scallop, Patinopecten yessoensis: Control by Estradiol-17β and the Central Nervous System'. Together they form a unique fingerprint.

Cite this