Visually induced initiation of Drosophila innate courtship-like following pursuit is mediated by central excitatory state

Soh Kohatsu, Daisuke Yamamoto

    Research output: Contribution to journalArticle

    57 Citations (Scopus)

    Abstract

    The courtship ritual of male Drosophila represents an innate behaviour that is initiated by female-derived sensory stimuli. Here we report that moving light spots can induce courtship-like following pursuit in tethered wild-type male flies provided the fly is primed by optogenetic stimulation of specific dsx-expressing neuronal clusters in the lateral protocerebrum (LPR). Namely, stimulation of the pC1 neuronal cluster initiates unilateral wing extension and vibration of both sides, whereas stimulation of the pC2l cluster initiates only contralateral wing displays. In addition, stimulation of pC2l but not pC1 neurons induced abdominal bending and proboscis extension. Ca 2+ imaging of the pC1 cluster revealed periodic Ca 2+ rises, each corresponding to a turn of the male fly during courtship. In contrast, group-reared fru mutant males exhibit light spot-induced courtship pursuit without optogenetic priming. Ca 2+ imaging revealed enhanced responses of LPR neurons to visual stimuli in the mutants, suggesting a neural correlate of the light spot-induced courtship behaviour.

    Original languageEnglish
    Article number6457
    JournalNature communications
    Volume6
    DOIs
    Publication statusPublished - 2015 Mar

    ASJC Scopus subject areas

    • Chemistry(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Physics and Astronomy(all)

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