Visualizing the spatiotemporal dynamics of DNA damage in budding yeast

Chihiro Horigome, Vincent Dion, Andrew Seeber, Lutz R. Gehlen, Susan M. Gasser

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Fluorescence microscopy has enabled the analysis of both the spatial distribution of DNA damage and its dynamics during the DNA damage response (DDR). Three microscopic techniques can be used to study the spatiotemporal dynamics of DNA damage. In the first part we describe how we determine the position of DNA double-strand breaks (DSBs) relative to the nuclear envelope. The second part describes how to quantify the co-localization of DNA DSBs with nuclear pore clusters, or other nuclear subcompartments. The final protocols describe methods for the quantification of locus mobility over time.

Original languageEnglish
Pages (from-to)77-96
Number of pages20
JournalMethods in Molecular Biology
Volume1292
DOIs
Publication statusPublished - 2015
Externally publishedYes

Keywords

  • Chromatin mobility
  • DNA damage
  • Double-strand breaks
  • Fluorescence microscopy
  • Mean-square displacement analysis
  • Nuclear pore clustering

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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