TY - JOUR
T1 - Visualization of the recovery process of defects in a cultured cell layer by chemical imaging sensor
AU - Miyamoto, Ko ichiro
AU - Yu, Bing
AU - Isoda, Hiroko
AU - Wagner, Torsten
AU - Schöning, Michael J.
AU - Yoshinobu, Tatsuo
N1 - Funding Information:
This work was supported by JSPS KAKENHI Grant Numbers 24310098 and 23615001. A part of this research was carried out at the Machine Shop Division of Fundamental Technology Center, Research Institute of Electrical Communication, Tohoku University. The authors are grateful to Dr. J. Han of Tsukuba University for his kind support and valuable suggestions.
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/11/29
Y1 - 2016/11/29
N2 - The chemical imaging sensor is a field-effect sensor which is able to visualize both the distribution of ions (in LAPS mode) and the distribution of impedance (in SPIM mode) in the sample. In this study, a novel cell assay is proposed, in which the chemical imaging sensor operated in SPIM mode is applied to monitor the recovery of defects in a cell layer brought into proximity of the sensing surface. A reduced impedance at a defect formed artificially in a cell layer was successfully visualized in a photocurrent image. The cell layer was cultured over two weeks, during which the temporal change of the photocurrent distribution corresponding to the recovery of the defect was observed.
AB - The chemical imaging sensor is a field-effect sensor which is able to visualize both the distribution of ions (in LAPS mode) and the distribution of impedance (in SPIM mode) in the sample. In this study, a novel cell assay is proposed, in which the chemical imaging sensor operated in SPIM mode is applied to monitor the recovery of defects in a cell layer brought into proximity of the sensing surface. A reduced impedance at a defect formed artificially in a cell layer was successfully visualized in a photocurrent image. The cell layer was cultured over two weeks, during which the temporal change of the photocurrent distribution corresponding to the recovery of the defect was observed.
KW - Cell migration assay
KW - Chemical imaging sensor
KW - Scanning photo-induced impedance microscopy
KW - Wound-healing assay
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U2 - 10.1016/j.snb.2016.04.018
DO - 10.1016/j.snb.2016.04.018
M3 - Article
AN - SCOPUS:84990046740
SN - 0925-4005
VL - 236
SP - 965
EP - 969
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
ER -