Visualization of somatic deletions mediated by R/RS site-specific recombination and induction of germinal deletions caused by callus differentiation and regeneration in rice

K. Toriyama, A. Chiba, Y. Nakagawa

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A transgenic rice plant expressing the recombinase of Zygosaccharomyces rouxii under the control of the CaMV 35S promoter was crossed with a transgenic plant carrying a cryptic (β-glucuronidase) GUS reporter gene, which was activated by recombinase-mediated deletions between two specific recombination sites (RSs). In F1 plants, GUS activity was observed as blue spots and stripes in vascular bundles in several parts of the leaves. GUS expression was detected in all of the calli induced from F1 seeds and throughout the regenerated plants. DNA analysis using the polymerase chain reaction and Southern blotting showed that R/RS-mediated deletions occurred in all of the cells of the regenerated plants. Stable GUS expression was confirmed in the progeny resulting from self-pollination. Thus, the deletions obtained in the regenerated plants were genetically equivalent to the germinal deletions. These results indicate that the induction of callus differentiation and shoot regeneration is an effective manner to activate the R/RS system and to produce plants with chromosomal deletions.

Original languageEnglish
Pages (from-to)605-610
Number of pages6
JournalPlant Cell Reports
Volume21
Issue number6
DOIs
Publication statusPublished - 2003 Feb 1

Keywords

  • Deletion
  • GUS
  • Oryza sativa L.
  • R/RS recombination system
  • Site-specific recombination

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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