Visualization of single membrane protein structure in stretched lipid bilayer suspended over nanowells

Youichi Shinozaki, Koji Sumitomo, Kazuaki Furukawa, Hidetoshi Miyashita, Yukihiro Tamba, Nahoko Kasai, Hiroshi Nakashima, Keiichi Torimitsu

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

In this study, we observed the topology of a single protein in a stretched lipid bilayer (membrane) suspended over a nanoscale well using a fast-scanning atomic force microscope (AFM). The membrane was located stably enough on the well to prevent the leakage of a liquid placed in the well, and it allowed us to observe membrane stretching using an AFM. We successfully observed the gradual stretching of the suspended membrane. We also observed single bacteriorhodopsin proteins in the stretched membrane, and found that they maintained their trimeric structure, but that the distances between the trimers increased.

Original languageEnglish
Article number027002
JournalApplied Physics Express
Volume3
Issue number2
DOIs
Publication statusPublished - 2010 Feb
Externally publishedYes

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

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