Visualization of Renal Microcirculation in Isolated Munich-Wistar Rat Kidneys: Effects of Endothelin-1 on Renal Hemodynamic Activity

Mamoru Saito, Isao Yamatsu, Satoshi Homma, Masaaki Sato, Norio Ohshima

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10 Citations (Scopus)

Abstract

The aim of the present study was to visualize the superficial glomeruli of the Munich-Wistar (MW) rat and to characterize the responses of the renal microvasculature to endothelin-1 (ET-1). We first examined the distribution of superficial glomeruli of the MW rat compared to that in a control strain (Wistar rat). Secondly, we examined the effects of ET-1 on the renal microcirculation of the MW rat. The right kidney was perfused with a Krebs-Ringer solution containing fluorescein isothiocyanate dextran (FITC-dex-tran) and was visualized under an epi-illuminated fluorescence microscope system. Changes in perfusion pressure and diameter of the microvessels accompanying the administration of ET-1 (10 fmole- 300 pmole) were measured. The number of superficial glomeruli was greater in the MW rat than in the Wistar rat. ET-1 had long-lasting and dose-dependent pressor effects. Perfusion pressure showed a 3.5-fold increase compared with the control, and the afferent arterioles showed greater dose-dependent vasoconstriction than the efferent arterioles. These findings suggest that the MW rat is a useful animal model for the study of renal microcirculation and that the renal microcirculation is extremely sensitive to ET-1.

Original languageEnglish
Pages (from-to)221-229
Number of pages9
JournalThe Japanese Journal of Pharmacology
Volume66
Issue number2
DOIs
Publication statusPublished - 1994

Keywords

  • Endothelin-1
  • Fluorescence microangiography
  • Intravital microscope system
  • Munich-Wistar rat
  • Renal microcirculation

ASJC Scopus subject areas

  • Pharmacology

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