Visualization of long-term Mg2+ dynamics in apoptotic cells using a novel targetable fluorescent probe

Yusuke Matsui, Yosuke Funato, Hiromi Imamura, Hiroaki Miki, Shin Mizukami, Kazuya Kikuchi

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Mg2+ plays important roles in many physiological processes. However, the underlying molecular mechanisms, especially in the apoptotic pathway, remain unclear due to the diffusion of Mg2+ probes, which hinders long-term imaging in specific organelles. We developed an immobilized Mg2+ probe, MGH, which is covalently conjugated with the HaloTag protein in various organelles. HaloTag-coupled MGH enabled long-term imaging of intracellular local Mg2+ dynamics for 24 h. To exploit this remarkable property, MGH was applied to the investigation of intracellular Mg2+ dynamics during apoptosis. Time-lapse imaging revealed an increase in the Mg2+ concentration after apoptotic cell shrinkage. Combined imaging analyses of intracellular Mg2+ and ATP concentrations strongly suggested that this Mg2+ concentration increase was caused by the dissociation of Mg2+ from ATP, along with a decrease in the intracellular ATP concentration. Thus, this protein-coupled Mg2+ probe could be a new chemical tool to elucidate intracellular Mg2+ dynamics with high spatiotemporal resolution.

Original languageEnglish
Pages (from-to)8255-8264
Number of pages10
JournalChemical Science
Volume8
Issue number12
DOIs
Publication statusPublished - 2017

ASJC Scopus subject areas

  • Chemistry(all)

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