Visualization of fluid drainage pathways in lymphatic vessels and lymph nodes using a mouse model to test a lymphatic drug delivery system

Tetsuya Kodama, Yuriko Hatakeyama, Shigeki Kato, Shiro Mori

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Curing/preventing micrometastasis to lymph nodes (LNs) located outside the surgically resected area is essential for improving the morbidity and mortality associated with breast cancer and head and neck cancer. However, no lymphatic therapy system exists that can deliver drugs to LNs located outside the dissection area. Here, we demonstrate proof of concept for a drug delivery system using MXH10/Mo-lpr/lpr mice that exhibit systemic lymphadenopathy, with some peripheral LNs being as large as 10 mm in diameter. We report that a fluorescent solution injected into the subiliac LN (defined as the upstream LN within the dissection area) was delivered successfully to the proper axillary LN (defined as the downstream LN outside the dissection area) through the lymphatic vessels. Our results suggest that this approach could be used before surgical resection to deliver drugs to downstream LNs outside the dissection area. We anticipate that our methodology could be applied clinically, before surgical resection, to cure/prevent micrometastasis in LNs outside the dissection area, using techniques such as ultrasound-guided internal jugular vein catheterization.

Original languageEnglish
Pages (from-to)124-134
Number of pages11
JournalBiomedical Optics Express
Volume6
Issue number1
DOIs
Publication statusPublished - 2015 Jan 1

Keywords

  • (170.0170) Medical optics and biotechnology
  • (170.3880) Medical and biological imaging
  • (170.5380) Physiology

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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