Visualization of EXGT-mediated molecular grafting activity by means of a fluorescent-labeled xyloglucan oligomer

Hisashi Ito, Kazuhiko Nishitani

    Research output: Contribution to journalArticlepeer-review

    42 Citations (Scopus)

    Abstract

    A fluoresceinyl xyloglucan heptasaccharide was synthesized and used as a probe to visualize the activity of the cell wall to incorporate xyloglucan fragments into the cellulose/xyloglucan framework in suspension-cultured tobacco cells. The activity was significantly reduced in transformant cells in which the expression of endoxyloglucan transferase (EXGT) was severely suppressed by over-expression of the antisense EXGT-N1 mRNA. These results indicate the involvement of EXGT in the construction of the cellulose/xyloglucan framework.

    Original languageEnglish
    Pages (from-to)1172-1176
    Number of pages5
    JournalPlant and Cell Physiology
    Volume40
    Issue number11
    DOIs
    Publication statusPublished - 1999

    Keywords

    • Antisense mRNA
    • Cell-wall construction
    • Cellulose/xyloglucan framework
    • Endoxyloglucan transferase
    • Fluoresceinyl xyloglucan oligomer
    • Tobacco BY-2 cell

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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