Visualization of EXGT-mediated molecular grafting activity by means of a fluorescent-labeled xyloglucan oligomer

Hisashi Ito, Kazuhiko Nishitani

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

A fluoresceinyl xyloglucan heptasaccharide was synthesized and used as a probe to visualize the activity of the cell wall to incorporate xyloglucan fragments into the cellulose/xyloglucan framework in suspension-cultured tobacco cells. The activity was significantly reduced in transformant cells in which the expression of endoxyloglucan transferase (EXGT) was severely suppressed by over-expression of the antisense EXGT-N1 mRNA. These results indicate the involvement of EXGT in the construction of the cellulose/xyloglucan framework.

Original languageEnglish
Pages (from-to)1172-1176
Number of pages5
JournalPlant and Cell Physiology
Volume40
Issue number11
DOIs
Publication statusPublished - 1999
Externally publishedYes

Keywords

  • Antisense mRNA
  • Cell-wall construction
  • Cellulose/xyloglucan framework
  • Endoxyloglucan transferase
  • Fluoresceinyl xyloglucan oligomer
  • Tobacco BY-2 cell

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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