TY - JOUR
T1 - Visualization of defects on a cultured cell layer by utilizing chemical imaging sensor
AU - Miyamoto, Ko Ichiro
AU - Bing, Yu
AU - Wagner, Torsten
AU - Yoshinobu, Tatsuo
AU - Schöning, Michael J.
N1 - Funding Information:
This study was supported by Grant-in-Aid for Scientific Research (C) (contract no. 23615001) from the Japan Society for the Promotion of Science (JSPS). A part of this research was carried out at the Machine Shop Division of Fundamental Technology Center, Research Institute of Electrical Communication, Tohoku University. Authors also acknowledge Dr. J. Han and Prof. Dr. H. Isoda of Tsukuba University for their kind support and suggestions.
Publisher Copyright:
© 2015 The Authors. Published by Elsevier Ltd.
PY - 2015
Y1 - 2015
N2 - The chemical imaging sensor is a field-effect sensor which is able to visualize both the distribution of ions (in LAPS mode) and the distribution of impedance (in SPIM mode) in the sample. In this study, a novel wound-healing assay is proposed, in which the chemical imaging sensor operated in SPIM mode is applied to monitor the defect of a cell layer brought into proximity of the sensing surface. A reduced impedance inside the defect, which was artificially formed in a cell layer, was successfully visualized in a photocurrent image.
AB - The chemical imaging sensor is a field-effect sensor which is able to visualize both the distribution of ions (in LAPS mode) and the distribution of impedance (in SPIM mode) in the sample. In this study, a novel wound-healing assay is proposed, in which the chemical imaging sensor operated in SPIM mode is applied to monitor the defect of a cell layer brought into proximity of the sensing surface. A reduced impedance inside the defect, which was artificially formed in a cell layer, was successfully visualized in a photocurrent image.
KW - Chemical imaging sensor
KW - SPIM, impedance, wound-healing assay
UR - http://www.scopus.com/inward/record.url?scp=84984984337&partnerID=8YFLogxK
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U2 - 10.1016/j.proeng.2015.08.806
DO - 10.1016/j.proeng.2015.08.806
M3 - Conference article
AN - SCOPUS:84984984337
VL - 120
SP - 936
EP - 939
JO - Procedia Engineering
JF - Procedia Engineering
SN - 1877-7058
T2 - 29th European Conference on Solid-State Transducers, EUROSENSORS 2015; Freiburg; Germany; 6 September 2015 through 9 September 2015.
Y2 - 6 September 2015 through 9 September 2015
ER -