Viscosity and drag force involved in organelle transport: Investigation of the fluctuation dissipation theorem

K. Hayashi, C. G. Pack, M. K. Sato, K. Mouri, K. Kaizu, K. Takahashi, Y. Okada

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We observed the motion of an organelle transported by motor proteins in cells using fluorescence microscopy. Particularly, among organelles, the mitochondria in PC12 cells were studied. A mitochondrion was dragged at a constant speed for several seconds without pausing. We investigated the fluctuation dissipation theorem for this constant drag motion by comparing it with the motion of Brownian beads that were incorporated into the cells by an electroporation method. We estimated the viscosity value inside cells from the diffusion coefficients of the beads. Then the viscosity value obtained by using the beads was found to be slightly lower than that obtained from the diffusion coefficient for the organelle motion via the Einstein relation. This discrepancy indicates the violation of the Einstein relation for the organelle motion.

Original languageEnglish
Article number136
JournalEuropean Physical Journal E
Volume36
Issue number12
DOIs
Publication statusPublished - 2013 Dec 1

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Chemistry(all)
  • Materials Science(all)
  • Surfaces and Interfaces

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