Virucidal effect of monogalactosyl diacylglyceride from a green microalga, Coccomyxa sp. KJ, against clinical isolates of SARS-CoV-2 as assessed by a plaque assay

Kyoko Hayashi, Satomi Asai, Kazuo Umezawa, Hidehumi Kakizoe, Hayato Miyachi, Masanobu Morita, Takaaki Akaike, Hitoshi Kuno, Satoko Komatsu, Takumi Watanabe, Toshio Kawahara

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19) and is capable of human-to-human transmission and rapid global spread. Thus, the establishment of high-quality viral detection and quantification methods, and the development of anti-SARS-CoV-2 agents are critical. Methods: Here, we present the rapid detection of infectious SARS-CoV-2 particles using a plaque assay with 0.5% agarose-ME (Medium Electroosmosis) as an overlay medium. Results: The plaques were capable of detecting the virus within 36–40 h post-infection. In addition, we showed that a monogalactosyl diacylglyceride isolated from a microalga (Coccomyxa sp. KJ) could inactivate the clinical isolates of SARS-CoV-2 in a time- and concentration-dependent manner. Conclusions: These results would allow rapid quantification of the infectious virus titers and help develop more potent virucidal agents against SARS-CoV-2.

Original languageEnglish
JournalJournal of Clinical Laboratory Analysis
DOIs
Publication statusAccepted/In press - 2021

Keywords

  • detection time
  • infectious virus
  • natural product
  • quantification
  • virucidal action

ASJC Scopus subject areas

  • Immunology and Allergy
  • Hematology
  • Public Health, Environmental and Occupational Health
  • Clinical Biochemistry
  • Medical Laboratory Technology
  • Biochemistry, medical
  • Microbiology (medical)

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