Virucidal effect of monogalactosyl diacylglyceride from a green microalga, Coccomyxa sp. KJ, against clinical isolates of SARS-CoV-2 as assessed by a plaque assay

Kyoko Hayashi; Satomi Asai; Kazuo Umezawa; Hidehumi Kakizoe; Hayato Miyachi; Masanobu Morita; Takaaki Akaike; Hitoshi Kuno; Satoko Komatsu; Takumi Watanabe; Toshio Kawahara

doi:10.1002/jcla.24146

Virucidal effect of monogalactosyl diacylglyceride from a green microalga, Coccomyxa sp. KJ, against clinical isolates of SARS-CoV-2 as assessed by a plaque assay

Kyoko Hayashi, Satomi Asai, Kazuo Umezawa, Hidehumi Kakizoe, Hayato Miyachi, Masanobu Morita, Takaaki Akaike, Hitoshi Kuno, Satoko Komatsu, Takumi Watanabe, Toshio Kawahara

MED - Public Health

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19) and is capable of human-to-human transmission and rapid global spread. Thus, the establishment of high-quality viral detection and quantification methods, and the development of anti-SARS-CoV-2 agents are critical. Methods: Here, we present the rapid detection of infectious SARS-CoV-2 particles using a plaque assay with 0.5% agarose-ME (Medium Electroosmosis) as an overlay medium. Results: The plaques were capable of detecting the virus within 36–40 h post-infection. In addition, we showed that a monogalactosyl diacylglyceride isolated from a microalga (Coccomyxa sp. KJ) could inactivate the clinical isolates of SARS-CoV-2 in a time- and concentration-dependent manner. Conclusions: These results would allow rapid quantification of the infectious virus titers and help develop more potent virucidal agents against SARS-CoV-2.

Original language	English
Journal	Journal of Clinical Laboratory Analysis
DOIs	https://doi.org/10.1002/jcla.24146
Publication status	Accepted/In press - 2021

Keywords

detection time
infectious virus
natural product
quantification
virucidal action

ASJC Scopus subject areas

Immunology and Allergy
Hematology
Public Health, Environmental and Occupational Health
Clinical Biochemistry
Medical Laboratory Technology
Biochemistry, medical
Microbiology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/jcla.24146

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Hayashi, K., Asai, S., Umezawa, K., Kakizoe, H., Miyachi, H., Morita, M., Akaike, T., Kuno, H., Komatsu, S., Watanabe, T., & Kawahara, T. (Accepted/In press). Virucidal effect of monogalactosyl diacylglyceride from a green microalga, Coccomyxa sp. KJ, against clinical isolates of SARS-CoV-2 as assessed by a plaque assay. Journal of Clinical Laboratory Analysis. https://doi.org/10.1002/jcla.24146

Virucidal effect of monogalactosyl diacylglyceride from a green microalga, Coccomyxa sp. KJ, against clinical isolates of SARS-CoV-2 as assessed by a plaque assay. / Hayashi, Kyoko; Asai, Satomi; Umezawa, Kazuo; Kakizoe, Hidehumi; Miyachi, Hayato; Morita, Masanobu; Akaike, Takaaki; Kuno, Hitoshi; Komatsu, Satoko; Watanabe, Takumi; Kawahara, Toshio.

In: Journal of Clinical Laboratory Analysis, 2021.

Research output: Contribution to journal › Article › peer-review

Hayashi, K, Asai, S, Umezawa, K, Kakizoe, H, Miyachi, H, Morita, M, Akaike, T, Kuno, H, Komatsu, S, Watanabe, T & Kawahara, T 2021, 'Virucidal effect of monogalactosyl diacylglyceride from a green microalga, Coccomyxa sp. KJ, against clinical isolates of SARS-CoV-2 as assessed by a plaque assay', Journal of Clinical Laboratory Analysis. https://doi.org/10.1002/jcla.24146

Hayashi, Kyoko ; Asai, Satomi ; Umezawa, Kazuo ; Kakizoe, Hidehumi ; Miyachi, Hayato ; Morita, Masanobu ; Akaike, Takaaki ; Kuno, Hitoshi ; Komatsu, Satoko ; Watanabe, Takumi ; Kawahara, Toshio. / Virucidal effect of monogalactosyl diacylglyceride from a green microalga, Coccomyxa sp. KJ, against clinical isolates of SARS-CoV-2 as assessed by a plaque assay. In: Journal of Clinical Laboratory Analysis. 2021.

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abstract = "Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19) and is capable of human-to-human transmission and rapid global spread. Thus, the establishment of high-quality viral detection and quantification methods, and the development of anti-SARS-CoV-2 agents are critical. Methods: Here, we present the rapid detection of infectious SARS-CoV-2 particles using a plaque assay with 0.5% agarose-ME (Medium Electroosmosis) as an overlay medium. Results: The plaques were capable of detecting the virus within 36–40 h post-infection. In addition, we showed that a monogalactosyl diacylglyceride isolated from a microalga (Coccomyxa sp. KJ) could inactivate the clinical isolates of SARS-CoV-2 in a time- and concentration-dependent manner. Conclusions: These results would allow rapid quantification of the infectious virus titers and help develop more potent virucidal agents against SARS-CoV-2.",

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author = "Kyoko Hayashi and Satomi Asai and Kazuo Umezawa and Hidehumi Kakizoe and Hayato Miyachi and Masanobu Morita and Takaaki Akaike and Hitoshi Kuno and Satoko Komatsu and Takumi Watanabe and Toshio Kawahara",

note = "Funding Information: This work was supported by JST Adaptable and Seamless Technology Transfer Program through Target‐driven R & D (A‐STEP) Grant Number JPMJTR204H. We would like to thank Editage ( www.editage.com ) for English language editing. Publisher Copyright: {\textcopyright} 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.",

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doi = "10.1002/jcla.24146",

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AU - Hayashi, Kyoko

AU - Asai, Satomi

AU - Umezawa, Kazuo

AU - Kakizoe, Hidehumi

AU - Miyachi, Hayato

AU - Morita, Masanobu

AU - Akaike, Takaaki

AU - Kuno, Hitoshi

AU - Komatsu, Satoko

AU - Watanabe, Takumi

AU - Kawahara, Toshio

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PY - 2021

Y1 - 2021

N2 - Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19) and is capable of human-to-human transmission and rapid global spread. Thus, the establishment of high-quality viral detection and quantification methods, and the development of anti-SARS-CoV-2 agents are critical. Methods: Here, we present the rapid detection of infectious SARS-CoV-2 particles using a plaque assay with 0.5% agarose-ME (Medium Electroosmosis) as an overlay medium. Results: The plaques were capable of detecting the virus within 36–40 h post-infection. In addition, we showed that a monogalactosyl diacylglyceride isolated from a microalga (Coccomyxa sp. KJ) could inactivate the clinical isolates of SARS-CoV-2 in a time- and concentration-dependent manner. Conclusions: These results would allow rapid quantification of the infectious virus titers and help develop more potent virucidal agents against SARS-CoV-2.

AB - Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19) and is capable of human-to-human transmission and rapid global spread. Thus, the establishment of high-quality viral detection and quantification methods, and the development of anti-SARS-CoV-2 agents are critical. Methods: Here, we present the rapid detection of infectious SARS-CoV-2 particles using a plaque assay with 0.5% agarose-ME (Medium Electroosmosis) as an overlay medium. Results: The plaques were capable of detecting the virus within 36–40 h post-infection. In addition, we showed that a monogalactosyl diacylglyceride isolated from a microalga (Coccomyxa sp. KJ) could inactivate the clinical isolates of SARS-CoV-2 in a time- and concentration-dependent manner. Conclusions: These results would allow rapid quantification of the infectious virus titers and help develop more potent virucidal agents against SARS-CoV-2.

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KW - infectious virus

KW - natural product

KW - quantification

KW - virucidal action

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Virucidal effect of monogalactosyl diacylglyceride from a green microalga, Coccomyxa sp. KJ, against clinical isolates of SARS-CoV-2 as assessed by a plaque assay

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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