TY - JOUR
T1 - Vestiges of adaptation to the mesophilic environment in the genome of tepiditoga spiralis gen. Nov., sp. nov.
AU - Mori, Koji
AU - Sakurai, Kenta
AU - Hosoyama, Akira
AU - Kakegawa, Takeshi
AU - Hanada, Satoshi
N1 - Publisher Copyright:
© 2020, Japanese Society of Microbial Ecology. All rights reserved.
PY - 2020
Y1 - 2020
N2 - A novel anaerobic heterotrophic strain, designated strain sy52T, was isolated from a hydrothermal chimney at Suiyo Seamount in the Pacific Ocean. A 16S rRNA gene analysis revealed that the strain belonged to the family Petrotogaceae in the phylum Thermotogae. The strain was mesophilic with optimum growth at 48°C and the phylum primarily comprised hyperthermophiles and thermophiles. Strain sy52T possessed unique genomic characteristics, such as an extremely low G+C content and 6 copies of rRNA operons. Genomic analyses of strain sy52T revealed that amino acid usage in the predicted proteins resulted from adjustments to mesophilic environments. Genomic features also indicated independent adaptions to the mesophilic environment of strain sy52T and Mesotoga species, which belong to the mesophilic lineage in the phylum Thermotogae. Based on phenotypic and phylogenetic evidence, strain sy52T is considered to represent a novel genus and species in the family Petrotogaceae with the proposed name Tepiditoga spiralis gen. nov., sp. nov.
AB - A novel anaerobic heterotrophic strain, designated strain sy52T, was isolated from a hydrothermal chimney at Suiyo Seamount in the Pacific Ocean. A 16S rRNA gene analysis revealed that the strain belonged to the family Petrotogaceae in the phylum Thermotogae. The strain was mesophilic with optimum growth at 48°C and the phylum primarily comprised hyperthermophiles and thermophiles. Strain sy52T possessed unique genomic characteristics, such as an extremely low G+C content and 6 copies of rRNA operons. Genomic analyses of strain sy52T revealed that amino acid usage in the predicted proteins resulted from adjustments to mesophilic environments. Genomic features also indicated independent adaptions to the mesophilic environment of strain sy52T and Mesotoga species, which belong to the mesophilic lineage in the phylum Thermotogae. Based on phenotypic and phylogenetic evidence, strain sy52T is considered to represent a novel genus and species in the family Petrotogaceae with the proposed name Tepiditoga spiralis gen. nov., sp. nov.
KW - Mesophilic environment
KW - Tepiditoga spiralis
KW - Thermotogae
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U2 - 10.1264/jsme2.ME20046
DO - 10.1264/jsme2.ME20046
M3 - Article
C2 - 32963207
AN - SCOPUS:85091189622
VL - 35
SP - 1
EP - 8
JO - Microbes and Environments
JF - Microbes and Environments
SN - 1342-6311
IS - 4
M1 - ME20046
ER -