Very low density lipoprotein receptor binds apolipoprotein E2/2 as well as apolipoprotein E3/3

Sadao Takahashi, Koji Oida, Minoru Ookubo, Jinya Suzuki, Mitsuyuki Kohno, Toshio Murase, Tokuo Yamamoto, Tsuguhiko Nakai

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

The VLDL receptor, a newly identified lipoprotein receptor, recognizes apoE containing lipoproteins. The human VLDL receptor was overexpressed in ldlA-7, a mutant Chinese hamster ovary cells lacking LDL receptors. Each VLDL obtained from a normolipidemic subject with two ε3 or ε2 alleles similarly competed for the binding of radiolabeled rabbit β-VLDL to the VLDL receptors. The anti-apoE monoclonal antibody 1D7, which inhibited binding of apoE3 to the LDL receptors, failed to compete for the binding of VLDL (apoE3 or apoE2) to the VLDL receptors. Results indicate that the binding site of apoE on the VLDL receptor may differ from its binding site on the LDL receptor.

Original languageEnglish
Pages (from-to)197-200
Number of pages4
JournalFEBS Letters
Volume386
Issue number2-3
DOIs
Publication statusPublished - 1996 May 20

Keywords

  • Apolipoprotein E
  • Lipoprotein
  • Lipoprotein receptor
  • VLDL receptor
  • Very low density lipoprotein

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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