Very late DNA replication in the human cell cycle

R. J. Widrow, R. Scott Hansen, Hiroshi Kawame, Stanley M. Gartler, Charles D. Laird

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33 Citations (Scopus)


G2 was defined originally as the temporal gap between the termination of DNA replication and the beginning of mitosis. In human cells, the G2 period was estimated to be 3-4 h. However, the absence of replicative DNA synthesis during this period designated G2 has never been shown conclusively. In this report, we show that, at some autosomal and X linked loci, programmed DNA replication continues within 90 min of mitosis. Furthermore, the major accumulation of cyclin B1, a cell-cycle marker that is usually ascribed to G2, overlaps extensively with very late DNA replication. We conclude that the G2 period is much shorter than previously thought and may, in some cells, be nonexistent.

Original languageEnglish
Pages (from-to)11246-11250
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number19
Publication statusPublished - 1998 Sep 15
Externally publishedYes

ASJC Scopus subject areas

  • General


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