Vasohibin-1 and retinal pigment epithelium

Yumi Ishikawa, Nobuhiro Nagai, Hideyuki Onami, Norihiro Kumasaka, Ryosuke Wakusawa, Hikaru Sonoda, Yasufumi Sato, Toshiaki Abe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Vascular endothelial growth factor (VEGF) is one of the main factors for inducing choroidal neovascularization in patients with age-related macular degeneration (AMD). Retinal pigment epithelium (RPE) is one of the main sources for expressing VEGF. Vasohibin-1 is a VEGF-inducible gene in human cultured endothelial cells with antiangiogenic properties. We examined the effects of vasohibin-1 against RPE. We used rat RPE cell line, RPE-J. Cobalt chloride and low glucose and oxygen supply were used for hypoxic stress. Western blot analysis and real-time PCR were performed to detect the expression of vasohibin-1 and VEGF in the RPE-J. Cobalt chloride or low oxygen and low glucose enhanced VEGF expression, whereas statistically significant less vasohibin-1 expression was observed. RPE cell dynamics was monitored using xCELLigence System for real-time cell analysis during culture. External VEGF (0.2-2 nM) enhanced Cell Index (CI), such as cell viability, number, and adhesion to the plates significantly at 2% oxygen and no glucose when compared to those of vehicle or other concentration of VEGF. Conversely, external vasohibin-1 (2 nM) showed lower CI at the indicated condition. Vasohibin-1 also reduced VEGF-induced CI index. These results were not observed under standard culture condition. When we performed MTS assay or cell count, external vasohibin-1 also showed comparable results. When we transduced full-length vasohibin-1 cDNA in RPE-J, the internal vasohibin-1 showed less VEGF expression than that of control vector-transduced RPE-J. In summary, vasohibin-1 showed opposite results as that of VEGF on the RPE-J, especially under hypoxic condition.

Original languageEnglish
Title of host publicationRetinal Degenerative Diseases
EditorsMatthew LaVail, Robert Anderson, Christian Grimm, John Ash, Joe Hollyfield
Pages305-310
Number of pages6
DOIs
Publication statusPublished - 2012

Publication series

NameAdvances in Experimental Medicine and Biology
Volume723
ISSN (Print)0065-2598

Keywords

  • Cell Dynamics
  • Cobalt Chloride
  • Hypoxia
  • Retinal Pigment Epithelium
  • VEGF
  • Vasohibin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Ishikawa, Y., Nagai, N., Onami, H., Kumasaka, N., Wakusawa, R., Sonoda, H., Sato, Y., & Abe, T. (2012). Vasohibin-1 and retinal pigment epithelium. In M. LaVail, R. Anderson, C. Grimm, J. Ash, & J. Hollyfield (Eds.), Retinal Degenerative Diseases (pp. 305-310). (Advances in Experimental Medicine and Biology; Vol. 723). https://doi.org/10.1007/978-1-4614-0631-0_40