Various spatiotemporal expression profiles of anther-expressed genes in rice

Tokunori Hobo, Keita Suwabe, Koichiro Aya, Go Suzuki, Kentaro Yano, Takeshi Ishimizu, Masahiro Fujita, Shunsuke Kikuchi, Kazuki Hamada, Masumi Miyano, Tomoaki Fujioka, Fumi Kaneko, Tomohiko Kazama, Yoko Mizuta, Hirokazu Takahashi, Katsuhiro Shiono, Mikio Nakazono, Nobuhiro Tsutsumi, Yoshiaki Nagamura, Nori KurataMasao Watanabe, Makoto Matsuoka

Research output: Contribution to journalArticlepeer-review

92 Citations (Scopus)

Abstract

The male gametophyte and tapetum play different roles during anther development although they are differentiated from the same cell lineage, the L2 layer. Until now, it has not been possible to delineate their transcriptomes due to technical difficulties in separating the two cell types. In the present study, we characterized the separated transcriptomes of the rice microspore/pollen and tapetum using laser microdissection (LM)-mediated microarray. Spatiotemporal expression patterns of 28,141 anther-expressed genes were classified into 20 clusters, which contained 3,468 (12.3%) anther-enriched genes. In some clusters, synchronous gene expression in the microspore and tapetum at the same developmental stage was observed as a novel characteristic of the anther transcriptome. Noteworthy expression patterns are discussed in connection with gene ontology (GO) categories and gene annotations, which are related to important biological events in anther development, such as pollen maturation, pollen germination, pollen tube elongation and pollen wall formation.

Original languageEnglish
Pages (from-to)1417-1428
Number of pages12
JournalPlant and Cell Physiology
Volume49
Issue number10
DOIs
Publication statusPublished - 2008 Oct

Keywords

  • Anther
  • Gene ontology
  • Laser microdissection
  • Microarray
  • Oryza sativa L.
  • Synchronous gene expression

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Various spatiotemporal expression profiles of anther-expressed genes in rice'. Together they form a unique fingerprint.

Cite this