Various roles of Th cytokine mRNA expression in different forms of Glomerulonephritis

Masakazu Ifuku, Katsuhisa Miyake, Maho Watanebe, Kenji Ito, Yasuhiro Abe, Yoshie Sasatomi, Satoru Ogahara, Satoshi Hisano, Hiroshi Sato, Takao Saito, Hitoshi Nakashima

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Background: Kidney disease is characterized by injurious immune responses to self or foreign antigens. The development and maintenance of immune responses generally involves activation of T lymphocytes. We evaluated mRNA expression patterns of T-cell cytokines to identify the principal Th-cell subset involved in the development of antineutrophil cytoplasmic antigen-associated pauci-immune crescentic glomerulonephritis (ANCAGN), membranoproliferative glomerulonephritis (MPGN), and membranous nephropathy (MN). Methods: Kidney biopsy specimens from ANCAGN (17), MPGN (11), and MN (14) patients were evaluated for mRNA expression of various T-cell cytokines. Results: Interferon-γ mRNA expression was detected in both ANCAGN and MPGN, but not in MN patients. Furthermore, mRNA expression of interleukin (IL)-12, a Th1-associated cytokine, was lower in MN patients than in ANCAGN and MPGN patients. In contrast, a significantly higher expression of IL-4 and IL-5 was observed in MN than in ANCAGN and MPGN patients. In the analyses of Th17-associated cytokine expression, a significantly higher expression of IL-6 and IL-17 was observed in ANCAGN than in MPGN and MN patients. No significant differences were observed in the expression of these cytokines between MPGN and MN patients. With regard to Treg-associated cytokines, a significantly higher IL-10 expression was observed in MN than in ANCAGN patients, and a significantly higher transforming growth factor-β expression was observed in MN than in ANCAGN and MPGN patients. Similarly, Foxp3 expression was significantly higher in MN. Conclusion: Th1 and Th17 immune responses in ANCAGN, the Th1 response in MPGN, and Th2 and Treg responses in MN patients may be integral for the distinct histological features of these diseases.

Original languageEnglish
Pages (from-to)115-123
Number of pages9
JournalAmerican Journal of Nephrology
Volume38
Issue number2
DOIs
Publication statusPublished - 2013 Aug

Keywords

  • Cytokine
  • Membranoproliferative glomerulonephritis
  • Membranous nephropathy
  • Pauci-immune crescentic glomerulonephritis
  • Th cell

ASJC Scopus subject areas

  • Nephrology

Fingerprint Dive into the research topics of 'Various roles of Th cytokine mRNA expression in different forms of Glomerulonephritis'. Together they form a unique fingerprint.

Cite this