Variations in 5’-Nucleotidase Activity in Established Mesenchymal Cell Lines from Syngeneic A/J Mice

Ryojin Uchino; Manabu Fukumoto; Toshihiro Adachi; Osamu Midorikawa

doi:10.1247/csf.8.119

Variations in 5’-Nucleotidase Activity in Established Mesenchymal Cell Lines from Syngeneic A/J Mice

Ryojin Uchino, Manabu Fukumoto, Toshihiro Adachi, Osamu Midorikawa

Research output: Contribution to journal › Article › peer-review

Abstract

5’-Nucleotidase activity was analyzed in four different mesenchymal cell lines (F, m, e and SP) established from syngeneic A/J mice. The 5’-nucleotidase activity of fibroblasts was lower in transformed cells (F and m) than in nontransformed cells (e). An increase in cell contact during confluence or during high cell density increased 5’-nucleotidase activity, and a decrease in cell contact caused a decrease in 5’-nucleotidase activity in both fibroblastic (F, m and e) and reticulum (SP) cell lines. These results are evidence that 5’-nucleotidase activity in mesenchymal cells is influenced by intercellular contact as well as transformation.

Original language	English
Pages (from-to)	119-126
Number of pages	8
Journal	Cell structure and function
Volume	8
Issue number	2
DOIs	https://doi.org/10.1247/csf.8.119
Publication status	Published - 1983 Jan
Externally published	Yes

ASJC Scopus subject areas

Physiology
Molecular Biology
Cell Biology

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title = "Variations in 5{\textquoteright}-Nucleotidase Activity in Established Mesenchymal Cell Lines from Syngeneic A/J Mice",

abstract = "5{\textquoteright}-Nucleotidase activity was analyzed in four different mesenchymal cell lines (F, m, e and SP) established from syngeneic A/J mice. The 5{\textquoteright}-nucleotidase activity of fibroblasts was lower in transformed cells (F and m) than in nontransformed cells (e). An increase in cell contact during confluence or during high cell density increased 5{\textquoteright}-nucleotidase activity, and a decrease in cell contact caused a decrease in 5{\textquoteright}-nucleotidase activity in both fibroblastic (F, m and e) and reticulum (SP) cell lines. These results are evidence that 5{\textquoteright}-nucleotidase activity in mesenchymal cells is influenced by intercellular contact as well as transformation.",

author = "Ryojin Uchino and Manabu Fukumoto and Toshihiro Adachi and Osamu Midorikawa",

year = "1983",

month = jan,

doi = "10.1247/csf.8.119",

language = "English",

volume = "8",

pages = "119--126",

journal = "Cell Structure and Function",

issn = "0386-7196",

publisher = "Japan Society for Cell Biology",

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T1 - Variations in 5’-Nucleotidase Activity in Established Mesenchymal Cell Lines from Syngeneic A/J Mice

AU - Uchino, Ryojin

AU - Fukumoto, Manabu

AU - Adachi, Toshihiro

AU - Midorikawa, Osamu

PY - 1983/1

Y1 - 1983/1

N2 - 5’-Nucleotidase activity was analyzed in four different mesenchymal cell lines (F, m, e and SP) established from syngeneic A/J mice. The 5’-nucleotidase activity of fibroblasts was lower in transformed cells (F and m) than in nontransformed cells (e). An increase in cell contact during confluence or during high cell density increased 5’-nucleotidase activity, and a decrease in cell contact caused a decrease in 5’-nucleotidase activity in both fibroblastic (F, m and e) and reticulum (SP) cell lines. These results are evidence that 5’-nucleotidase activity in mesenchymal cells is influenced by intercellular contact as well as transformation.

AB - 5’-Nucleotidase activity was analyzed in four different mesenchymal cell lines (F, m, e and SP) established from syngeneic A/J mice. The 5’-nucleotidase activity of fibroblasts was lower in transformed cells (F and m) than in nontransformed cells (e). An increase in cell contact during confluence or during high cell density increased 5’-nucleotidase activity, and a decrease in cell contact caused a decrease in 5’-nucleotidase activity in both fibroblastic (F, m and e) and reticulum (SP) cell lines. These results are evidence that 5’-nucleotidase activity in mesenchymal cells is influenced by intercellular contact as well as transformation.

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Variations in 5’-Nucleotidase Activity in Established Mesenchymal Cell Lines from Syngeneic A/J Mice

Abstract

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