Variations and Distributions of O-Glycosidically Linked Sugar Chains in Bovine κ-Casein

Tadao Saito, Takatoshi Itoh

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107 Citations (Scopus)

Abstract

The variation and distribution of O-glycosidically linked sugar chains in κ-casein from bovine mature milk were analyzed by HPLC, fast atom bombardment mass spectrometry, and 13C nuclear magnetic resonance. A monosaccharide alditol (N-acetylgalactosaminitol) and four oligosaccharide alditols [a neutral disaccharide (galactosyl-β1-3N-acetylgalactosaminitol), two acidic trisaccharides (straight chain: N-acetylneuraminyl α2-3galactosyl-β1-3N-acetylgalactosaminitol and branch chain: galactosyl β1-3 (N-acetylneuraminyl α2-6)N-acetylgalactosaminitol), and an acidic tetrasaccharide (N-acetylneuraminyl α2-3galactosyl-β1-3(N-acetylgalactosaminyl α2-6)N-acetylgalactosaminitol] were identified as sugar chains linking on normal bovine κ-casein. The most dominant sugar chain was an acidic tetrasaccharide. The four oligosaccharide chains were identical to those already found, but N-acetylgalactosamine was a newly identified sugar chain linking on κ-casein. The distribution of monosaccharide, disaccharide, trisaccharide (straight), trisaccharide (branched), and tetrasaccharide chains were determined by HPLC to be .8, 6.3, 18.4, 18.5, and 56.0%, respectively (means of five κ-caseins).

Original languageEnglish
Pages (from-to)1768-1774
Number of pages7
JournalJournal of Dairy Science
Volume75
Issue number7
DOIs
Publication statusPublished - 1992

Keywords

  • C nuclear magnetic resonance
  • CGP
  • CNMR
  • FABMS
  • Gal
  • Gal-NAc
  • GalNAc
  • N-acetylgalactosamhe
  • N-acetylgalactosaminitol
  • N-acetylneuraminyl
  • NANA
  • carbon-13 nuclear magnetic resonance
  • caseinoglycopeptide (κ-casein, f106–l69)
  • fast atom bombardment mass spectrometry
  • fast atom bombardment mass spectrometry
  • galactosyl
  • sugar chain distribution
  • κ-casein

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

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