Variations and Distributions of O-Glycosidically Linked Sugar Chains in Bovine κ-Casein

Tadao Saito, Takatoshi Itoh

    Research output: Contribution to journalArticlepeer-review

    128 Citations (Scopus)

    Abstract

    The variation and distribution of O-glycosidically linked sugar chains in κ-casein from bovine mature milk were analyzed by HPLC, fast atom bombardment mass spectrometry, and 13C nuclear magnetic resonance. A monosaccharide alditol (N-acetylgalactosaminitol) and four oligosaccharide alditols [a neutral disaccharide (galactosyl-β1-3N-acetylgalactosaminitol), two acidic trisaccharides (straight chain: N-acetylneuraminyl α2-3galactosyl-β1-3N-acetylgalactosaminitol and branch chain: galactosyl β1-3 (N-acetylneuraminyl α2-6)N-acetylgalactosaminitol), and an acidic tetrasaccharide (N-acetylneuraminyl α2-3galactosyl-β1-3(N-acetylgalactosaminyl α2-6)N-acetylgalactosaminitol] were identified as sugar chains linking on normal bovine κ-casein. The most dominant sugar chain was an acidic tetrasaccharide. The four oligosaccharide chains were identical to those already found, but N-acetylgalactosamine was a newly identified sugar chain linking on κ-casein. The distribution of monosaccharide, disaccharide, trisaccharide (straight), trisaccharide (branched), and tetrasaccharide chains were determined by HPLC to be .8, 6.3, 18.4, 18.5, and 56.0%, respectively (means of five κ-caseins).

    Original languageEnglish
    Pages (from-to)1768-1774
    Number of pages7
    JournalJournal of Dairy Science
    Volume75
    Issue number7
    DOIs
    Publication statusPublished - 1992

    Keywords

    • C nuclear magnetic resonance
    • CGP
    • CNMR
    • FABMS
    • Gal
    • Gal-NAc
    • GalNAc
    • N-acetylgalactosamhe
    • N-acetylgalactosaminitol
    • N-acetylneuraminyl
    • NANA
    • carbon-13 nuclear magnetic resonance
    • caseinoglycopeptide (κ-casein, f106–l69)
    • fast atom bombardment mass spectrometry
    • fast atom bombardment mass spectrometry
    • galactosyl
    • sugar chain distribution
    • κ-casein

    ASJC Scopus subject areas

    • Food Science
    • Animal Science and Zoology
    • Genetics

    Fingerprint

    Dive into the research topics of 'Variations and Distributions of O-Glycosidically Linked Sugar Chains in Bovine κ-Casein'. Together they form a unique fingerprint.

    Cite this