Abstract
Photoautotrophic fatty acid production of a highly CO2-tolerant green alga Chlorococcum littorale in the presence of inorganic carbon at 295 K and light intensity of 170 μmol-photon m-2 s-1 was investigated. CO2 concentration in the bubbling gas was adjusted by mixing pure gas components of CO2 and N2 to avoid photorespiration and β-oxidation of fatty acids under O2 surrounding conditions. Maximum content of total fatty acid showed pH-dependence after nitrate depletion of the culture media and increased with the corresponding inorganic carbon ratio. Namely, [HCO3-]/([CO2]+n[ CO32-]) ratio in the culture media was found to be a controlling factor for photoautotrophic fatty acid production after the nitrate limitation. At a CO2 concentration of 5% (vol/vol) and a pH of 6.7, the fatty acid content was 47.8 wt % (dry basis) at its maximum that is comparable with land plant seed oils.
Original language | English |
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Pages (from-to) | 1053-1057 |
Number of pages | 5 |
Journal | Biotechnology Progress |
Volume | 31 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2015 Jul 1 |
Keywords
- Algal oil
- Biofuels
- Biomass
- Green algae
- Microalgae
ASJC Scopus subject areas
- Biotechnology