Variation of photoautotrophic fatty acid production from a highly CO2 tolerant alga, Chlorococcum littorale, with inorganic carbon over narrow ranges of pH

Masaki Ota, Motohiro Takenaka, Yoshiyuki Sato, Richard L. Smith, Hiroshi Inomata

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Photoautotrophic fatty acid production of a highly CO2-tolerant green alga Chlorococcum littorale in the presence of inorganic carbon at 295 K and light intensity of 170 μmol-photon m-2 s-1 was investigated. CO2 concentration in the bubbling gas was adjusted by mixing pure gas components of CO2 and N2 to avoid photorespiration and β-oxidation of fatty acids under O2 surrounding conditions. Maximum content of total fatty acid showed pH-dependence after nitrate depletion of the culture media and increased with the corresponding inorganic carbon ratio. Namely, [HCO3-]/([CO2]+n[ CO32-]) ratio in the culture media was found to be a controlling factor for photoautotrophic fatty acid production after the nitrate limitation. At a CO2 concentration of 5% (vol/vol) and a pH of 6.7, the fatty acid content was 47.8 wt % (dry basis) at its maximum that is comparable with land plant seed oils.

Original languageEnglish
Pages (from-to)1053-1057
Number of pages5
JournalBiotechnology Progress
Volume31
Issue number4
DOIs
Publication statusPublished - 2015 Jul 1

Keywords

  • Algal oil
  • Biofuels
  • Biomass
  • Green algae
  • Microalgae

ASJC Scopus subject areas

  • Biotechnology

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