Variation in the pattern of [Ca2+]i change induced by acetylcholine in cultured hippocampal neurons

Toshio Iijima, Yoshihisa Kudo, Akihiko Ogura, Kyoto Akita, Gen Matsumoto

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)

    Abstract

    Acetylcholine (ACh) caused various patterns of change in the intracellular Ca2+ concentration ([Ca2+]i) in cultured rat hippocampal neurons. We studied the underlying mechanisms of the [Ca2+]i changes with simultaneous recording of [Ca2+ i and membrane potential/current. In most cases, [Ca2+]i rise was accompanied by a membrane depolarization. The [Ca2+]i change was significantly reduced when the membrane was voltage clamped, which implies that most of the [Ca2+]i rise results from the Ca2+ influx through the voltate-gated Ca2+ channel activated by the membrane depolarization. The membrane depolarizations were classified into two types, one associated with membrane conductance decrease and the other associated with membrane conductance increase. The former results from potassium conductance ((gK+) decrease, and the latter may result from the activation of a Na+-permeable channel. However, [Ca2+]i elevation was also observed in some neurons showing membrane hyperpolarization in response to ACh. This seems to show that ACh liberates Ca2+ from the intracellular Ca2+ store, resulting in the activation of a calcium-dependent K+ channel (KCa). The variations of ACh in the hippocampal neurons seem to result from a variety of muscarinic acetylcholine receptors and various species of ion channels governed by those receptors.

    Original languageEnglish
    Pages (from-to)273-280
    Number of pages8
    JournalBrain research
    Volume521
    Issue number1-2
    DOIs
    Publication statusPublished - 1990 Jun 25

    Keywords

    • Acetylcholine
    • Calcium-dependent K-channel
    • Hippocampal neuron
    • Inositol triphosphate
    • Intracellular calcium
    • M-channel

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Molecular Biology
    • Clinical Neurology
    • Developmental Biology

    Fingerprint Dive into the research topics of 'Variation in the pattern of [Ca<sup>2+</sup>]<sub>i</sub> change induced by acetylcholine in cultured hippocampal neurons'. Together they form a unique fingerprint.

    Cite this