Variation in lysine, threonine, and tryptophan availability in meat and bone meal as estimated by the slope-ratio growth assay technique in growing rats

Hiroyuki Sato, Makoto Miura, Takeshi Fujieda, Marcin Taciak, Motoi Kikusato, Masaaki Toyomizu

Research output: Contribution to journalArticlepeer-review

Abstract

Meat and bone meal (MBM) is an important protein source used in animal feeds. However, as the composition and availability of amino acids (AAs) in MBM fluctuate markedly, it is important to verify the magnitude of these parameters in different MBMs. In this study, the AA compositions of 19 MBMs were analyzed to confirm variations in lysine (Lys), threonine (Thr), and tryptophan (Trp), then which were compared with those of soybean meal (SBM) and fish meal (FM). Instability in Lys, Thr, and Trp availabilities in six MBMs were also considered after estimation using the slope-ratio growth assay technique in rats. Variations in AA composition were evaluated using the coefficient of variance (CV: Standard deviation/Mean). CVs for Lys, Thr, and Trp content were 9.40, 11.83, and 18.12 in MBM, 2.71, 2.48, and 3.19 in SBM, and 10.09, 10.44, and 13.47 in FM. Furthermore, means and SDs for Lys, Thr, and Trp availabilities in MBM were 53.3 ± 10.4% (CV: 19.5), 65.9 ± 17.6% (CV: 26.6), and 83.2 ± 11.2% (CV: 13.5), respectively. These results provide the first evidence that variations in MBM AA compositions were 3.5 to 5.7 times higher than those in SBM, but similar to FM, and that the large variation in availability substantially existed.

Original languageEnglish
Article numbere13413
JournalAnimal Science Journal
Volume91
Issue number1
DOIs
Publication statusPublished - 2020 Jan 1

Keywords

  • AA availability
  • lysine
  • meat and bone meal
  • threonine
  • tryptophan

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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