The distribution of polyprenyl pyrophosphates synthesized by the action of solanesyl pyrophosphate synthetase from Micrococcus luteus is dramatically changed depending on the Mg++ concentration. When the metal ion concentration is higher than 5 mM, octaprenyl and solanesyl (nonaprenyl) pyrophosphate are synthesized predominantly. On the other hand, when the metal ion level is lower than 0.5 mM, a variety of polyprenyl pyrophosphates ranging in carbon chain length from C15 to C40 are formed. Heptaprenyl pyrophosphate is the longest of the products formed at 0.1 mM of Mg++.
- high pressure liquid chromatography
ASJC Scopus subject areas
- Molecular Biology