Variable gene segment-specific N-insertions at the signal joint of T- cell receptor Vβ-Dβ recombinations

Yasuyoshi Kanari, Ryusuke Nakagawa, Hiroshi Arakawa, Hideo Yamagishi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The V(D)J recombination of immunoglobulin and a T-cell receptor generates two species of DNA junctions, a coding joint and a signal joint. Non-templated nucleotides (N-nucleotides) are inserted in these DNA junctions. We analyzed the N-insertion at signal joints generated by the Vβ- Dβ recombinations. N-insertions were detected at signal joints of Vβ2, Vβ3, Vβ10, Vβ18 and Vβ14 but not in Vβ8 and Vβ7. These data show that the N-insertion at signal joints is dependent on the Vβ locus used for the recombination. We suggest that the regional chromosomal configuration may differ in recombinase accessibility.

Original languageEnglish
Pages (from-to)151-155
Number of pages5
JournalImmunology Letters
Volume61
Issue number2-3
DOIs
Publication statusPublished - 1998 Apr

Keywords

  • N-insertion
  • Signal joint
  • T-cell receptor β
  • V-DJ recombination

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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