VAMP4 is required to maintain the ribbon structure of the Golgi apparatus

Akiko Shitara, Toru Shibui, Miki Okayama, Toshiya Arakawa, Itaru Mizoguchi, Yasunori Shakakura, Taishin Takuma

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

The Golgi apparatus forms a twisted ribbon-like network in the juxtanuclear region of vertebrate cells. Vesicle-associated membrane protein 4 (VAMP4), a v-SNARE protein expressed exclusively in the vertebrate trans-Golgi network (TGN), plays a role in retrograde trafficking from the early endosome to the TGN, although its precise function within the Golgi apparatus remains unclear. To determine whether VAMP4 plays a functional role in maintaining the structure of the Golgi apparatus, we depleted VAMP4 gene expression using RNA interference technology. Depletion of VAMP4 from HeLa cells led to fragmentation of the Golgi ribbon. These fragments were not uniformly distributed throughout the cytoplasm, but remained in the juxtanuclear area. Electron microscopy and immunohistochemistry showed that in the absence of VAMP4, the length of the Golgi stack was shortened, but Golgi stacking was normal. Anterograde trafficking was not impaired in VAMP4-depleted cells, which contained intact microtubule arrays. Depletion of the cognate SNARE partners of VAMP4, syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure. Our findings suggested that the maintenance of Golgi ribbon structure requires normal retrograde trafficking from the early endosome to the TGN, which is likely to be mediated by the formation of VAMP4-containing SNARE complexes.

Original languageEnglish
Pages (from-to)11-21
Number of pages11
JournalMolecular and Cellular Biochemistry
Volume380
Issue number1-2
DOIs
Publication statusPublished - 2013 Aug
Externally publishedYes

Keywords

  • Golgi fragmentation
  • Golgi ribbon structure
  • Membrane transport
  • RNAi
  • SNARE
  • VAMP4

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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