Vamp2 marks quiescent satellite cells and myotubes, but not activated myoblasts

Yuki Tajika, Maiko Takahashi, Mizuki Hino, Tohru Murakami, Hiroshi Yorifuji

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

We examined the expression and intrallular localization of vesicle-associated membrane protein 2 (VAMP2) during the differentiation of skeletal muscle cells by immunofluorescence microscopy. In isolated single myofibers, VAMP2 was expressed in quiescent satellite cells, downregulated in proliferating myoblastic cells, and re-expressed with differentiation. In the myoblastic cell line C2C12, VAMP2 was expressed at a low level in the proliferating stage, and then increased after differentiation into myotubes. Based on these results, we propose that VAMP2 can be used as a molecular marker for both quiescent satellite cells and myotubes, but not for proliferating myoblasts. We also found the partial colocalization of VAMP2 with transferrin- or Rab11-labeled vesicles in myotubes, suggesting a role of VAMP2 in the trafficking of recycling endosomes. 2010 The Japan Society of Histochemistry and Cytochemistry.

Original languageEnglish
Pages (from-to)107-114
Number of pages8
JournalACTA HISTOCHEMICA ET CYTOCHEMICA
Volume43
Issue number4
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • C2c12 cells
  • Myotube
  • Satellite cell
  • Snare
  • Vamp

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

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