TY - JOUR
T1 - Value of three-dimensional maximum intensity projection display to assist in magnetic resonance imaging (MRI)-based grading in a mouse model of subarachnoid hemorrhage
AU - Mutoh, Tomoko
AU - Mutoh, Tatsushi
AU - Sasaki, Kazumasu
AU - Nakamura, Kazuhiro
AU - Taki, Yasuyuki
AU - Ishikawa, Tatsuya
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of ScienceJSPS (15K10966), Life Science Foundation of Japan, and an Institutional Research Grant from Akita Prefecture
Publisher Copyright:
© Med Sci Monit, 2016.
Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 2016/6/16
Y1 - 2016/6/16
N2 - Background: Subarachnoid hemorrhage (SAH) is one of the most devastating cerebrovascular disorders. We report on the diagnostic value of three-dimensional (3-D) maximum intensity projection (MIP) reconstruction of T2*-weighted magnetic resonance images (MRI), processed using graphical user interface-based software, to aid in the accurate grading of endovascular-perforation-induced SAH in a mouse model. Material/Methods: A total of 30 mice were subjected to SAH by endovascular perforation; three (10%) were scored as grade 0, six (20%) as grade 1, six (20%) as grade 2, eight (27%) as grade 3, and seven (23%) as grade 4 according to T2*-weighted coronal slices. In comparison, none of mice were scored as grade 0, eight (27%) as grade 1, five (17%) as grade 2, nine (30%) as grade 3, and eight (27%) as grade 4 based on subsequent evaluation using reconstructed 3-D MIP images. Results: Mice scored as grade 0 (10%; no visible SAH) on T2*-coronal images were categorized as grades 1 (thin/localized SAH) and 3 (thick/diffuse SAH) according to 3-D MIP images. Grades based on T2* 3-D MIP images were more closely correlated with conventional SAH score (r2=0.59; P<0.0001) and neurological score (r2=0.25; P=0.005) than those based on T2*-coronal slices (r2=0.46; P<0.0001 for conventional score and r2=0.15; P=0.035 for neurological score). Conclusions: These results suggest that 3-D MIP images generated from T2*-weighted MRI data may be useful for the simple and precise grading of SAH severity in mice to overcome the weakness of the current MRI-based SAH grading system.
AB - Background: Subarachnoid hemorrhage (SAH) is one of the most devastating cerebrovascular disorders. We report on the diagnostic value of three-dimensional (3-D) maximum intensity projection (MIP) reconstruction of T2*-weighted magnetic resonance images (MRI), processed using graphical user interface-based software, to aid in the accurate grading of endovascular-perforation-induced SAH in a mouse model. Material/Methods: A total of 30 mice were subjected to SAH by endovascular perforation; three (10%) were scored as grade 0, six (20%) as grade 1, six (20%) as grade 2, eight (27%) as grade 3, and seven (23%) as grade 4 according to T2*-weighted coronal slices. In comparison, none of mice were scored as grade 0, eight (27%) as grade 1, five (17%) as grade 2, nine (30%) as grade 3, and eight (27%) as grade 4 based on subsequent evaluation using reconstructed 3-D MIP images. Results: Mice scored as grade 0 (10%; no visible SAH) on T2*-coronal images were categorized as grades 1 (thin/localized SAH) and 3 (thick/diffuse SAH) according to 3-D MIP images. Grades based on T2* 3-D MIP images were more closely correlated with conventional SAH score (r2=0.59; P<0.0001) and neurological score (r2=0.25; P=0.005) than those based on T2*-coronal slices (r2=0.46; P<0.0001 for conventional score and r2=0.15; P=0.035 for neurological score). Conclusions: These results suggest that 3-D MIP images generated from T2*-weighted MRI data may be useful for the simple and precise grading of SAH severity in mice to overcome the weakness of the current MRI-based SAH grading system.
KW - Image processing, computer-assisted
KW - Magnetic resonance imaging
KW - Mice, c57bl/6
KW - Subarachnoid hemorrhage
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U2 - 10.12659/MSM.896499
DO - 10.12659/MSM.896499
M3 - Article
C2 - 27307024
AN - SCOPUS:84977475342
VL - 22
SP - 2050
EP - 2055
JO - Medical Science Monitor
JF - Medical Science Monitor
SN - 1234-1010
ER -