Vaccination with tumor cell lysate-pulsed dendritic cells augments the effect of IFN-β gene therapy for malignant glioma in an experimental mouse intracranial glioma

Ryuta Saito, Masaaki Mizuno, Norimoto Nakahara, Takaya Tsuno, Toshihiro Kumabe, Takashi Yoshimoto, Jun Yoshida

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Interferon-β (IFN-β) has been used as an antitumor drug against human glioma, melanoma and medulloblastoma since the 1980s. Recently, we developed a new gene therapy using the IFN-β gene against malignant gliomas and then began clinical trials in 2000. Since stimulation of immune system was one mechanism of antitumor effect induced by IFN-β gene therapy, we hypothesized that combination of IFN-β gene therapy with immunotherapy might increase its effectiveness. In the present study, we tested whether combination therapy with IFN-β gene therapy and immunotherapy using tumor cell lysate-pulsed dendritic cells (DCs) would increase the efficacy of IFN-β gene therapy. In an experimental mouse intracranial glioma (GL261), which cannot be cured by either IFN-β gene therapy or DC immunotherapy alone, IFN-β gene therapy following DC immunotherapy resulted in a significant prolongation in survival of the mice. Moreover, when this combination was performed twice, 50% of treated mice survived longer than 100 days. Considering these results, this combination therapy may be one promising candidate for glioma therapy in the near future.

Original languageEnglish
Pages (from-to)777-782
Number of pages6
JournalInternational Journal of Cancer
Volume111
Issue number5
DOIs
Publication statusPublished - 2004 Sep 20

Keywords

  • Dendritic cells
  • Gene therapy
  • Glioma
  • Immunotherapy
  • Interferon-β gene

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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