Abstract
Stably transfected PC12D cell lines overexpressing a catecholamine biosynthesis regulatory protein, V-1, were used to examine the functional role of V-1 in catecholamine secretion. High K+-induced dopamine secretion in V-1 overexpressing clones was shown to be markedly potentiated compared with control clones carried with a vector alone. As assayed intracellular calcium concentration ([Ca2+]i) using fura-PE3, V-1 overexpression was observed to enhance high K+-elicited [Ca2+]i elevation. Electron microscopic analysis revealed an increase in dense-cored vesicle formation by V-1 overexpression. These results suggest that the enhancement of high K+-induced dopamine secretion by V-1 overexpression results from the potentiation of high K+-induced [Ca2+]i elevation and the increase in the number of dense-cored vesicles.
Original language | English |
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Pages (from-to) | 94-98 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 530 |
Issue number | 1-3 |
DOIs | |
Publication status | Published - 2002 Oct 23 |
Keywords
- Dopamine biosynthesis
- Dopamine secretion
- Exocytosis
- Overexpression
- PC12D cells
- V-1
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology