V-1, a catecholamine biosynthesis regulatory protein, positively controls catecholamine secretion in PC12D cells

Tohru Yamakuni, Toshifumi Yamamoto, Yukisato Ishida, Hideko Yamamoto, Si Young Song, Eijiro Adachi, Yusuke Hiwatashi, Yasushi Ohizumi

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Stably transfected PC12D cell lines overexpressing a catecholamine biosynthesis regulatory protein, V-1, were used to examine the functional role of V-1 in catecholamine secretion. High K+-induced dopamine secretion in V-1 overexpressing clones was shown to be markedly potentiated compared with control clones carried with a vector alone. As assayed intracellular calcium concentration ([Ca2+]i) using fura-PE3, V-1 overexpression was observed to enhance high K+-elicited [Ca2+]i elevation. Electron microscopic analysis revealed an increase in dense-cored vesicle formation by V-1 overexpression. These results suggest that the enhancement of high K+-induced dopamine secretion by V-1 overexpression results from the potentiation of high K+-induced [Ca2+]i elevation and the increase in the number of dense-cored vesicles.

Original languageEnglish
Pages (from-to)94-98
Number of pages5
JournalFEBS Letters
Volume530
Issue number1-3
DOIs
Publication statusPublished - 2002 Oct 23

Keywords

  • Dopamine biosynthesis
  • Dopamine secretion
  • Exocytosis
  • Overexpression
  • PC12D cells
  • V-1

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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