UV-induced reactivation and mutagenesis of λ-phages after treatment with 8-Methoxypsoralen or thiopyronine and light

Akira Yasui, Klaus Winckler, Wolfgang Laskowski

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    Lesions, which were produced on λ-phages DNA by the photosensitization effect of 8-methoxypsoralen (8-MOP) can be repaired by UV-induced repair systems (W-reactivation) in Escherichia coli wild type host cells. By optimum induction of the repair system, about 45% of the 8-MOP lesions are repaired. The survival of λ-phages inactivated by the photodynamic action of thiopyronine (TP) is only negligibly increased by the same UV-induced repair systems. However, the frequencies of clear plaque mutations of 8-MOP treated as well as TP treated λ-phages increase in similar fashion if the host cells of wild type have been irradiated with UV. These results show the different capacities of induced repair systems in repairing different types of lesions. They also suggest that some types of base damages are repaired more error-prone than other DNA-lesions.

    Original languageEnglish
    Pages (from-to)239-245
    Number of pages7
    JournalRadiation and Environmental Biophysics
    Volume19
    Issue number4
    DOIs
    Publication statusPublished - 1981 Dec 1

    ASJC Scopus subject areas

    • Biophysics
    • Radiation
    • Environmental Science(all)

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