UV-B signalling in rice: Response identification, gene expression profiling and mutant isolation

Muhammad Idris, Nobu Seo, Lei Jiang, Seiichiro Kiyota, Jun Hidema, Moritoshi Iino

Research output: Contribution to journalArticlepeer-review

Abstract

Responses of rice seedlings to UV-B radiation (UV-B) were investigated, aiming to establish rice as a model plant for UV-B signalling studies. The growth of japonica rice coleoptiles, grown under red light, was inhibited by brief irradiation with UV-B, but not with blue light. The effective UV-B fluences (10−1-103 μmol m−2) were much lower than those reported in Arabidopsis. The response was much less in indica rice cultivars and its extent varied among Oryza species. We next identified UV-B-specific anthocyanin accumulation in the first leaf of purple rice and used this visible phenotype to isolate mutants. Some isolated mutants were further characterized, and one was found to have a defect in the growth response. Using microarrays, we identified a number of genes that are regulated by low-fluence-rate UV-B in japonica coleoptiles. Some up-regulated genes were analysed by real-time PCR for UV-B specificity and the difference between japonica and indica. More than 70% of UV-B-regulated rice genes had no homologs in UV-B-regulated Arabidopsis genes. Many UV-B-regulated rice genes are related to plant hormones and especially to jasmonate biosynthetic and responsive genes in apparent agreement with the growth response. Possible involvement of two rice homologs of UVR8, a UV-B photoreceptor, is discussed.

Original languageEnglish
Pages (from-to)1468-1485
Number of pages18
JournalPlant Cell and Environment
Volume44
Issue number5
DOIs
Publication statusPublished - 2021 May

Keywords

  • Oryza sativa L.
  • anthocyanin
  • coleoptile
  • first leaf
  • growth inhibition
  • photomorphogenesis, plant hormone, photoreceptor
  • purple rice
  • rice phylogeny

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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