UV-B-induced CPD photolyase gene expression is regulated by UVR8-dependent and-independent pathways in arabidopsis

Nan Li, Mika Teranishi, Hiroko Yamaguchi, Tomonao Matsushita, Masaaki K. Watahiki, Tomohiko Tsuge, Shao Shan Li, Jun Hidema

    Research output: Contribution to journalArticlepeer-review

    25 Citations (Scopus)

    Abstract

    Plants have evolved various mechanisms that protect against the harmful effects of UV-B radiation (280-315 nm) on growth and development. Cyclobutane pyrimidine dimer (CPD) photolyase, the repair enzyme for UV-B-induced CPDs, is essential for protecting cells from UV-B radiation. Expression of the CPD photolyase gene (PHR) is controlled by light with various wavelengths including UV-B, but the mechanisms of this regulation remain poorly understood. In this study, we investigated the regulation of PHR expression by light with various wavelengths, in particular low-fluence UV-B radiation (280 nm, 0.2 μmol m-2 s-1), in Arabidopsis thaliana seedlings grown under light-dark cycles for 7 d and then adapted to the dark for 3 d. Low-fluence UV-B radiation induced CPDs but not reactive oxygen species. AtPHR expression was effectively induced by UV-B, UV-A (375 nm) and blue light. Expression induced by UV-A and blue light was predominantly regulated by the cryptochrome-dependent pathway, whereas phytochromes A and B played a minor but noticeable role. Expression induced by UV-B was predominantly regulated by the UVR8-dependent pathway. AtPHR expression was also mediated by a UVR8-independent pathway, which is correlated with CPD accumulation induced by UV-B radiation. These results indicate that Arabidopsis has evolved diverse mechanisms to regulate CPD photolyase expression by multiple photoreceptor signaling pathways, including UVR8-dependent and -independent pathways, as protection against harmful effects of UV-B radiation.

    Original languageEnglish
    Pages (from-to)2014-2023
    Number of pages10
    JournalPlant and Cell Physiology
    Volume56
    Issue number10
    DOIs
    Publication statusPublished - 2015 Jan 8

    Keywords

    • Arabidopsis
    • CPD photolyase
    • Cryptochrome
    • Cyclobutane pyrimidine dimer (CPD)
    • Gene expression
    • UVR8

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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