Utilization of the porcine system to study lymphotoxin-β regulation in intestinal lymphoid tissue

Tomoyuki Shimazu, Masanori Tohno, Shinichiro Katoh, Takeshi Shimosato, Hisashi Aso, Yasushi Kawai, Tadao Saito, Haruki Kitazawa

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Lymphotoxin-β (LT-β) has been suggested to be a regulator of secondary lymphoid structure development. In the present study, we isolated porcine LT-β (poLT-β) from adult swine spleens. The open reading frame encoded a predicted 246-amino acid polypeptide exhibiting higher similarity to the human than the mouse LT-β protein. Expression of LT-β mRNA in various swine tissues was analyzed by real-time PCR, and it was found to be higher in the ileal Peyer's patches (Pps) of adults than in newborns. In addition, ligand stimulation of toll-like receptors 2, 4, and 9, which are activated by bacterial components, increased LT-β expression only in neonatal ileal Pps. These results suggest that colonization by commensal bacteria may affect the maturation of neonatal ileal Pps by the induction of LT-β via toll-like receptors. LT-β may therefore be useful for studying the development of the intestinal immune system at parturition in both swine and humans.

Original languageEnglish
Pages (from-to)126-136
Number of pages11
JournalBiochemical Genetics
Volume47
Issue number1-2
DOIs
Publication statusPublished - 2009 Feb

Keywords

  • GALT
  • LT-β porcine
  • Neonate
  • Toll-like receptors

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry
  • Molecular Biology
  • Genetics

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