Utilization of recombinant Trichoderma reesei expressing Aspergillus aculeatus β-glucosidase I (JN11) for a more economical production of ethanol from lignocellulosic biomass

Treesukon Treebupachatsakul, Koki Shioya, Hikaru Nakazawa, Takashi Kawaguchi, Yasushi Morikawa, Yosuke Shida, Wataru Ogasawara, Hirofumi Okada

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

The capacity of Trichoderma reesei cellulase to degrade lignocellulosic biomass has been enhanced by the construction of a recombinant T. reesei strain expressing Aspergillus aculeatus β-glucosidase I. We have confirmed highly efficient ethanol production from converge-milled Japanese cedar by recombinant T. reesei expressing A. aculeatus β-glucosidase I (JN11). We investigated the ethanol productivity of JN11 and compared it with the cocktail enzyme T. reesei PC-3-7 with reinforced cellobiase activity by the commercial Novozyme 188. Results showed that the ethanol production efficiency under enzymatic hydrolysis of JN11 was comparable to the cocktail enzyme both on simultaneous saccharification and fermentation (SSF) or separate hydrolysis and fermentation (SHF) processes. Moreover, the cocktail enzyme required more protein loading for attaining similar levels of ethanol conversion as JN11. We propose that JN11 is an intrinsically economical enzyme that can eliminate the supplementation of BGL for PC-3-7, thereby reducing the cost of industrial ethanol production from lignocellulosic biomass.

Original languageEnglish
Pages (from-to)657-665
Number of pages9
JournalJournal of Bioscience and Bioengineering
Volume120
Issue number6
DOIs
Publication statusPublished - 2015 Dec
Externally publishedYes

Keywords

  • Aspergillus aculeatus BGL I
  • Ethanol
  • Separate hydrolysis and fermentation
  • Simultaneous saccharification and fermentation
  • Trichoderma reesei

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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